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DNA extraction - Salting Out: Difference between revisions
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*You may wish to kill the Proteinase K (95°C, 10 minutes) after the extraction though if you are proceding straight to PCR (and don't leave your reactions sitting around before thermal cycling) the denaturation step will take care of that | *You may wish to kill the Proteinase K (95°C, 10 minutes) after the extraction though if you are proceding straight to PCR (and don't leave your reactions sitting around before thermal cycling) the denaturation step will take care of that | ||
*Successfully used on fish, mammal and insect tissue. | *Successfully used on fish, mammal and insect tissue. | ||
*Works well in 96 well plate format, to remove ethanol following precipitation/wash simply invert the plate over a sink then spin gently (~100 rcf) inverted over absorbant paper. | |||
==Acknowledgments== | ==Acknowledgments== | ||
