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DNA extraction - Salting Out: Difference between revisions
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==Curators== | ==Curators== | ||
Marcus McHale [[Special:Emailuser/Marcus McHale|Email me through OpenWetWare]] | *Marcus McHale [[Special:Emailuser/Marcus McHale|Email me through OpenWetWare]] | ||
*This protocol has evolved with me and I am sure many others, please share your optimisations. | |||
''Anyone should feel free to add themselves as a curator for this consensus protocol. You do not need to be a curator in order to contribute. The OpenWetWare community is currently [[OpenWetWare:Information management/Protocol curators|discussing the idea of protocol curators]]. Please contribute.'' | ''Anyone should feel free to add themselves as a curator for this consensus protocol. You do not need to be a curator in order to contribute. The OpenWetWare community is currently [[OpenWetWare:Information management/Protocol curators|discussing the idea of protocol curators]]. Please contribute.'' | ||
==Abstract== | ==Abstract== | ||
Simple procedure for DNA extraction using salt | *Simple procedure for DNA extraction using salt | ||
==Materials== | ==Materials== | ||
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==Critical steps== | ==Critical steps== | ||
*After adding sodium acetate and centrifuging be careful not to transfer any of the white solid (SDS) into the fresh tube | |||
*Ensure to dry the pelletted DNA completely before attempting to resuspend | |||
==Troubleshooting== | ==Troubleshooting== | ||
==Notes== | ==Notes== | ||
*You may wish to kill the Proteinase K (95°C, 10 minutes) after the extraction though if you are proceding straight to PCR (and don't leave your reactions sitting around before thermal cycling) the denaturation step will take care of that | |||
==Acknowledgments== | ==Acknowledgments== | ||
*Damien Broderick from Queensland Depaqrtment of Primary Industries and Fisheries supplied me with his version of this protocol to start me off. | |||
==References== | ==References== | ||
Miller SA, Dykes DD, Polesky HF (1988) A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Research, 16, 1215–1215. | *Miller SA, Dykes DD, Polesky HF (1988) A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Research, 16, 1215–1215. | ||
==Specific Protocols== | ==Specific Protocols== | ||
