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Electrocompetent cells: Difference between revisions
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==Notes== | ==Notes== | ||
*'''[[User:Jason R. Kelly|Jason R. Kelly]] 15:14, 13 July 2007 (EDT):''' You might consider using a syringe filter rather than spins to do the washes when preparing the cells. This can apparently save a lot of time, though I've yet to try it myself. | *'''[[User:Jason R. Kelly|Jason R. Kelly]] 15:14, 13 July 2007 (EDT):''' You might consider using a syringe filter rather than spins to do the washes when preparing the cells. This can apparently save a lot of time, though I've yet to try it myself. | ||
*'''[[User:Hugh Kingston|hugh kingston]] 08:09, 17 April 2008 (EDT)''': I tried with a 0.22μm syringe filter and 50ml OD 600 e.coli. The filter kept jamming, not very successful. Perhaps a vacuum filter would be better? | |||
[[Category:Protocol]] | [[Category:Protocol]] | ||
[[Category:In vivo]] | [[Category:In vivo]] | ||
[[Category:Escherichia coli]] | [[Category:Escherichia coli]] | ||
