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QRT-PCR: Difference between revisions
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== Primer selection == | == Primer selection == | ||
=== Primer repositories === | |||
=== Primer repositories and collections === | |||
*[http://medgen.ugent.be/rtprimerdb/ RTPrimerDB] is an excellent database storing known primer and probe sequences for popular techniques (SYBR Green I, Taqman, Hybridisation Probes, Molecular Beacon). This can help you save the time of designing and testing your own primers. It is also intended to facilitate standardisation among different laboratories. The database is hosted by the Center for Medical Genetics, Gent, Belgium. Please submit you tested primer pairs. | *[http://medgen.ugent.be/rtprimerdb/ RTPrimerDB] is an excellent database storing known primer and probe sequences for popular techniques (SYBR Green I, Taqman, Hybridisation Probes, Molecular Beacon). This can help you save the time of designing and testing your own primers. It is also intended to facilitate standardisation among different laboratories. The database is hosted by the Center for Medical Genetics, Gent, Belgium. Please submit you tested primer pairs. | ||
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*[http://primerdepot.nci.nih.gov/ qPrimerDepot]. From the website "''This database provides qRT PCR primers for 99.96% human RefSeq sequences. For 99% of intron-bearing genes, the PCR product will cross an exon-exon border which overlaps one of the largest introns. All primers have annealing temperatures approximately 60C''". Link to [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1635330 paper]. | *[http://primerdepot.nci.nih.gov/ qPrimerDepot]. From the website "''This database provides qRT PCR primers for 99.96% human RefSeq sequences. For 99% of intron-bearing genes, the PCR product will cross an exon-exon border which overlaps one of the largest introns. All primers have annealing temperatures approximately 60C''". Link to [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1635330 paper]. | ||
* Check out the Eccles Lab collection of human and mouse [[Eccles:QPCR_reference_genes| qPCR reference genes]] on OWW. | |||
=== Primer design === | === Primer design === | ||
