Knight:DNA ligation using T4 DNA ligase: Difference between revisions

Knight:DNA ligation using T4 DNA ligase (view source)

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	#''Optional'' Heat-inactivate by incubating at 65°C for 20 mins. Then do a [[Purification of DNA \| purification]] step to remove PEG. (See notes on [[DNA Ligation\|DNA ligation]].		#''Optional'' Heat-inactivate by incubating at 65°C for 20 mins. Then do a [[Purification of DNA \| purification]] step to remove PEG. (See notes on [[DNA Ligation\|DNA ligation]].

	[[Category:DNA]] [[Category:In vitro]]		[[Category:DNA]] [[Category:In vitro]] [[Category:Protocol]]