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Testing with all combinations of cis/trans elements is currently on-going with YFP. In addition, recent work with cis3 plus trans1 and trans2 has shown a significant increase in fluorescence (from ~1% of unregulated to ~15%). In the near future, the cis3 construct will be transferred to regulate Q expression. Successful repression and activation of Q at threshold levels for the switch from lysis to lysogeny will allow for integration of teh riboregulated Q gene to be inserted into lambda phage. | Testing with all combinations of cis/trans elements is currently on-going with YFP. In addition, recent work with cis3 plus trans1 and trans2 has shown a significant increase in fluorescence (from ~1% of unregulated to ~15%). In the near future, the cis3 construct will be transferred to regulate Q expression. Successful repression and activation of Q at threshold levels for the switch from lysis to lysogeny will allow for integration of teh riboregulated Q gene to be inserted into lambda phage. | ||
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#isaacs pmid=15208640 | |||
</biblio> | |||
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