96
edits
IGEM:Caltech/2007/Project/Riboregulator: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
IGEM:Caltech/2007/Project/Riboregulator (view source)
Revision as of 19:28, 26 October 2007
, 26 October 2007→Status and Future Plans
| Line 62: | Line 62: | ||
Control plasmids with no cis repressive elements showed increased YFP fluorescence with no aTc (2.5%) and with 1ug/mL aTc (71.7%). The YFP gene under TetR control was used as the 100% marker. Plasmids with cis-repressive elements were grown in cells under the same conditions. The cis7 cultures showed repression of YFP fluorescence at 0.1ug/mL aTc(1.1%) and at 1ug/mL aTC (3.7%). The cis3 repressor showed 2.9% fluorescence and cis4 showed 0.84% fluorescence at 1ug/mL aTc. Repression was observed for all variants measured. | Control plasmids with no cis repressive elements showed increased YFP fluorescence with no aTc (2.5%) and with 1ug/mL aTc (71.7%). The YFP gene under TetR control was used as the 100% marker. Plasmids with cis-repressive elements were grown in cells under the same conditions. The cis7 cultures showed repression of YFP fluorescence at 0.1ug/mL aTc(1.1%) and at 1ug/mL aTC (3.7%). The cis3 repressor showed 2.9% fluorescence and cis4 showed 0.84% fluorescence at 1ug/mL aTc. Repression was observed for all variants measured. | ||
'''Future Plans''' | |||
Testing with all combinations of cis/trans elements is currently on-going with YFP. In addition, recent work with cis3 plus trans1 and trans2 has shown a significant increase in fluorescence (from ~1% of unregulated to ~15%). In the near future, the cis3 construct will be transferred to regulate Q expression. Successful repression and activation of Q at threshold levels for the switch from lysis to lysogeny will allow for integration of teh riboregulated Q gene to be inserted into lambda phage. | |||
|} | |} | ||
</div> | </div> | ||
