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IGEM:Caltech/2007/Project/Riboregulator: Difference between revisions
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IGEM:Caltech/2007/Project/Riboregulator (view source)
Revision as of 19:18, 26 October 2007
, 26 October 2007→Status and Future Plans
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'''Initial Results with Cis Repressors''' | '''Initial Results with Cis Repressors''' | ||
Control plasmids with no cis repressive elements showed increased YFP fluorescence with no aTc (2.5%) and with 1ug/mL aTc (71.7%). The YFP gene under TetR control was used as the 100% marker. Plasmids with cis-repressive elements were grown in cells under the same conditions. The cis7 cultures showed repression of YFP fluorescence at 0.1ug/mL (1.1%) and at 1ug/mL (3.7%). The cis3 repressor showed 2.9% fluorescence and cis4 showed 0.84% fluorescence at 1ug/mL aTc. Repression was observed for all variants measured. | Control plasmids with no cis repressive elements showed increased YFP fluorescence with no aTc (2.5%) and with 1ug/mL aTc (71.7%). The YFP gene under TetR control was used as the 100% marker. Plasmids with cis-repressive elements were grown in cells under the same conditions. The cis7 cultures showed repression of YFP fluorescence at 0.1ug/mL aTc(1.1%) and at 1ug/mL aTC (3.7%). The cis3 repressor showed 2.9% fluorescence and cis4 showed 0.84% fluorescence at 1ug/mL aTc. Repression was observed for all variants measured. | ||
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