IGEM:Caltech/2007/Project/Riboregulator: Difference between revisions

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Revision as of 19:12, 26 October 2007

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→‎Riboregulator Design
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[[Image:Cis1 4 and trans1 2.JPG|left|thumb|500px|The cis1-cis4 repressive riboregulators are shown with their corresponding activators, trans1 and trans2. All structures differ in the number of base-pairs in end-loops, number of bulges, and number of base pairs on the 5' toe-hold. They were designed this way to find the optimal dynamic range in repression/activation of protein expression.]]
[[Image:Cis1 4 and trans1 2.JPG|left|thumb|500px|The cis1-cis4 repressive riboregulators are shown with their corresponding activators, trans1 and trans2. All structures differ in the number of base-pairs in end-loops, number of bulges, and number of base pairs on the 5' toe-hold. They were designed this way to find the optimal dynamic range in repression/activation of protein expression.]]


[[Image: Cis568 trans3.JPG|left|thumb|800px|The cis5,6 and 8 repressive riboregulators are shown with their corresponding activators, trans3.]]
[[Image: Cis568 trans3.JPG|right|thumb|500px|The cis5,6 and 8 repressive riboregulators are shown with their corresponding activators, trans3.]]


[[Image:Cis7 trans4.JPG|left|thumb|600px|The cis7 repressor was the only structure that gave an unfavorable activating structure with trans3 from the cis5-8 set; thus, a separate trans structure was designed to specifically target cis7.]]
[[Image:Cis7 trans4.JPG|left|thumb|500px|The cis7 repressor was the only structure that gave an unfavorable activating structure with trans3 from the cis5-8 set; thus, a separate trans structure was designed to specifically target cis7.]]


[[Image: Cis5678 trans5.JPG|right|thumb|800px|The trans5 riboregulator was designed differently from trans1-4 in that it should bind to the 5’ end of the cis regulator and open up the hairpin in the 3’ direction. Bimolecular structures with cis5-8 all showed favorable activation in terms of free energies.]]
[[Image: Cis5678 trans5.JPG|right|thumb|500px|The trans5 riboregulator was designed differently from trans1-4 in that it should bind to the 5’ end of the cis regulator and open up the hairpin in the 3’ direction. Bimolecular structures with cis5-8 all showed favorable activation in terms of free energies.]]


[[Image:Cis trans compliments.JPG|right|thumb|500px|A schematic diagram showing the parts of cis1 repressor and trans1 activator that are complimentary to each other. Note that the RBS is now open.]]
[[Image:Cis trans compliments.JPG|right|thumb|500px|A schematic diagram showing the parts of cis1 repressor and trans1 activator that are complimentary to each other. Note that the RBS is now open.]]
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