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IGEM:Caltech/2007/Project/Riboregulator: Difference between revisions
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IGEM:Caltech/2007/Project/Riboregulator (view source)
Revision as of 19:02, 26 October 2007
, 26 October 2007→Riboregulator Design
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[[Image:Cis designs 1.JPG|left|thumb|800px|All 8 cis-repressive riboregulators have similar structure but differ in the number of base-pairs in end-loops, number of bulges, and number of base pairs on the 5' toe. They were designed this way to find the optimal dynamic range in repression/activation of protein expression.]] | [[Image:Cis designs 1.JPG|left|thumb|800px|All 8 cis-repressive riboregulators have similar structure but differ in the number of base-pairs in end-loops, number of bulges, and number of base pairs on the 5' toe. They were designed this way to find the optimal dynamic range in repression/activation of protein expression.]] | ||
[[Image:Cis1 4 and trans1 2.JPG| | [[Image:Cis1 4 and trans1 2.JPG|left|thumb|800px|The cis1-cis4 repressive riboregulators are shown with their corresponding activators, trans1 and trans2. All structures differ in the number of base-pairs in end-loops, number of bulges, and number of base pairs on the 5' toe-hold. They were designed this way to find the optimal dynamic range in repression/activation of protein expression.]] | ||
[[Image: Cis568 trans3.JPG| | [[Image: Cis568 trans3.JPG|left|thumb|800px|The cis5,6 and 8 repressive riboregulators are shown with their corresponding activators, trans3.]] | ||
[[Image:Cis7 trans4.JPG|left|thumb|600px|The cis7 repressor was the only structure that gave an unfavorable activating structure with trans3 from the cis5-8 set; thus, a separate trans structure was designed to specifically target cis7.]] | |||
[[Image:Cis trans compliments.JPG|right|thumb|500px|A schematic diagram showing the parts of cis1 repressor and trans1 activator that are complimentary to each other. Note that the RBS is now open.]] | [[Image:Cis trans compliments.JPG|right|thumb|500px|A schematic diagram showing the parts of cis1 repressor and trans1 activator that are complimentary to each other. Note that the RBS is now open.]] | ||
