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IGEM:Caltech/2007/Project/Riboregulator: Difference between revisions
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IGEM:Caltech/2007/Project/Riboregulator (view source)
Revision as of 08:11, 26 October 2007
, 26 October 2007→Experiments with Riboregulators
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In order to have a working system in which phage infection outcome is controlled, the expression of genes under the control of a riboregulator must be determined. Once the amount of N, Q, or Cro necessary to switch the system from lysis to lysogeny (or nothing) is determined, an appropriate riboregulator can be integrated into the system which allows for the correct amount of protein to be made in the trans-activating cells. | In order to have a working system in which phage infection outcome is controlled, the expression of genes under the control of a riboregulator must be determined. Once the amount of N, Q, or Cro necessary to switch the system from lysis to lysogeny (or nothing) is determined, an appropriate riboregulator can be integrated into the system which allows for the correct amount of protein to be made in the trans-activating cells. | ||
Before combining the phage protein expression with the riboregulator part of the system, it is necessary to engineer various riboregulators and determine the percent of protein ( | Before combining the phage protein expression with the riboregulator part of the system, it is necessary to engineer various riboregulators and determine the percent of protein (yellow fluorescent protein or YFP) in the presence and absence of trans RNA. By quantifying the riboregulated GFP system, we could then apply the results to each protein. | ||
==Status and Future Plans== | ==Status and Future Plans== | ||
