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IGEM:Caltech/2007/Project/Riboregulator: Difference between revisions
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IGEM:Caltech/2007/Project/Riboregulator (view source)
Revision as of 08:09, 26 October 2007
, 26 October 2007→Riboregulator Integration in Our Phage/''E. coli'' System
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==Riboregulator Integration in Our Phage/''E. coli'' System== | ==Riboregulator Integration in Our Phage/''E. coli'' System== | ||
The riboregulator determines what happens to the cell once it is infected by phage. We aimed to design three types of riboregulated systems (one for each protein N, Q, and Cro). For instance, for the N protein-regulated system, the phage genome would have a mutant version of the N gene as well as an N gene suppressed with a cis-repressive sequence would be additionally integrated into the genome. When the phage infects a wild-type E.coli cell, nothing should happen. However, when it infects a cell with the trans-activating | The riboregulator determines what happens to the cell once it is infected by phage. We aimed to design three types of riboregulated systems (one for each protein N, Q, and Cro). For instance, for the N protein-regulated system, the phage genome would have a mutant version of the N gene as well as an N gene suppressed with a cis-repressive sequence would be additionally integrated into the genome. When the phage infects a wild-type E.coli cell, nothing should happen. However, when it infects a cell with the trans-activating noncoding RNA, the cell should lyse because the RBS of the N gene is no longer sequestered by the cis-repressed RNA loop. | ||
==Riboregulator Design== | ==Riboregulator Design== | ||
