SOC

Summary

SOC Medium. Used in bacterial transformations

Ingredients

• 0.5% (w/v) yeast extract
• 2% (w/v) tryptone
• 10 mM NaCl
• 2.5 mM KCl
• 10 mM MgCl₂
• 20 mM MgSO₄
• 20 mM glucose

Protocol

1. SOB (SOC - glucose) is available at the kitchen
2. Add 1/100 volume 40% glucose (also available from the kitchen)

Source

Adapted From:

F. Ausubel et al., Short Protocols in Molecular Biology (John Wiley & Sons, ed. 4, 1999) pg. A1-36