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Annealing complementary primers: Difference between revisions
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Annealing complementary primers (view source)
Revision as of 13:56, 29 September 2005
, 29 September 2005no edit summary
Barry Canton (talk | contribs) No edit summary |
Barry Canton (talk | contribs) No edit summary |
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*For the annealing mix one recipe that works is as follows - | *For the annealing mix one recipe that works is as follows - | ||
** | **8 μL of each of the concentrated primers. | ||
**4 μL of salt solution (10 mM NaCl) | **4 μL of salt solution (10 mM NaCl) | ||
** | **20 μL of water | ||
*The salt shields the negative charges on the single-stranded DNA molecules, allowing them to come close enough to bind. | *The salt shields the negative charges on the single-stranded DNA molecules, allowing them to come close enough to bind. | ||
