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Preparing chemically competent cells: Difference between revisions
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==Using== | ==Using== | ||
#Thaw TSS cells on ice. | #Thaw TSS cells on ice. | ||
#Add DNA, pipette gently to mix. | #Add DNA, pipette gently to mix (1μl of prepped plasmid is more than enough). | ||
#Let sit 30 minutes on ice. | #Let sit for 30 minutes on ice. | ||
#Incubate cells for 30 seconds at <math>42^o</math>C. | #Incubate cells for 30 seconds at <math>42^o</math>C. | ||
#Add 1 mL [[SOC]] ( | #Add 1 mL [[SOC]] ([[2XYT]] is also suitable) at room temp. | ||
#Incubate for 1 hour at <math>37^o</math>C. | #Incubate for 1 hour at <math>37^o</math>C. | ||
#Plate 200 µL onto appropriate | #Plate 200 µL onto plate with appropriate antibiotic. | ||
