Sauer:Lysing E. coli with Lysozymes: Difference between revisions

In my construct, I placed the phage Lambda lysozyme "R gene" under constitutive control of the moderate Bla promoter from beta-lactamase.  This is a promoter that appears to exhibit even expression throughout growth (reference coming, some RNA micro-array paper).  Cells harboring this plasmid grow well and can be frozen if there is at least 10% glycerol present.  To lyse these cells, I add 10 uL of chloroform per mL of liquid and vortex.  At room temp, the cells completely lyse in about a minute.