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Purification of DNA: Difference between revisions
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→Alcohol precipitation: comparison EtOH 2PrOH
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#[[Isopropanol Precipitation for PCR Purification]] | #[[Isopropanol Precipitation for PCR Purification]] | ||
#*use to concentrate DNA from PCR when you have verified that only a single PCR product exists. When performed properly this procedure exceeds the recovery of QIAquick PCR purification columns | #*use to concentrate DNA from PCR when you have verified that only a single PCR product exists. When performed properly this procedure exceeds the recovery of QIAquick PCR purification columns | ||
2-propanol (isopropanol) precipitation yields less DNA than EtOH precipitation. (In my hands 60% less on average! [[User:Jasu|Jasu]]) | |||
2-propanol at RT reduces the risk of co-precipitation of salts (which can interfere with downstream experiments). 2-propanol is preferred for large volumes of DNA since less alcohol is required, plus it's faster since it doesn't require a cooling step. Glassy pellets from 2-propanol precipitation are harder to see than fluffy, salt-containing pellets from ethanol precipitation. 2-propanol pellets are also more loosely attached after centrifugation. Take care when decanting. | |||
==Filtration / affinity columns== | ==Filtration / affinity columns== | ||
