Purification of DNA: Difference between revisions
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There are several methods for purifying DNA. The one you choose depends on the nature of your DNA sample and your downstream applications. | There are several methods for purifying DNA. The one you choose depends on the nature of your DNA sample and your downstream applications. | ||
==Alcohol precipitation== | |||
#[[Ethanol precipitation of nucleic acids]] | #[[Ethanol precipitation of nucleic acids]] | ||
#[[Ethanol precipitation of small DNA fragments]] | #[[Ethanol precipitation of small DNA fragments]] | ||
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#*use to concentrate DNA from PCR when you have verified that only a single PCR product exists. When performed properly this procedure exceeds the recovery of QIAquick PCR purification columns | #*use to concentrate DNA from PCR when you have verified that only a single PCR product exists. When performed properly this procedure exceeds the recovery of QIAquick PCR purification columns | ||
==Filtration / affinity columns== | |||
#[[Centrifugal filtration/Nucleic acids]] | #[[Centrifugal filtration/Nucleic acids]] | ||
#*for small DNA fragments (~50-200 bp in length) | #*for small DNA fragments (~50-200 bp in length) | ||
Revision as of 11:00, 25 April 2007
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There are several methods for purifying DNA. The one you choose depends on the nature of your DNA sample and your downstream applications.
Alcohol precipitation
- Ethanol precipitation of nucleic acids
- Ethanol precipitation of small DNA fragments
- Isopropanol Precipitation for PCR Purification
- use to concentrate DNA from PCR when you have verified that only a single PCR product exists. When performed properly this procedure exceeds the recovery of QIAquick PCR purification columns
Filtration / affinity columns
- Centrifugal filtration/Nucleic acids
- for small DNA fragments (~50-200 bp in length)
- removes proteins, nucleotides and salts
- Miniprep
- for purifying plasmid DNA from E. coli cells
- QIAquick PCR purification
- for typical DNA fragments (> 200 bp in length)
