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Purification of DNA: Difference between revisions
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grouped protocols according to method
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There are several methods for purifying DNA. The one you choose depends on the nature of your DNA sample and your downstream applications. | There are several methods for purifying DNA. The one you choose depends on the nature of your DNA sample and your downstream applications. | ||
===Alcohol precipitation=== | |||
#[[Nucleic acid precipitation]] | |||
#[[Ethanol precipitation of small DNA fragments]] | #[[Ethanol precipitation of small DNA fragments]] | ||
#[[Isopropanol Precipitation for PCR Purification]] | |||
#*use to concentrate DNA from PCR when you have verified that only a single PCR product exists. When performed properly this procedure exceeds the recovery of QIAquick PCR purification columns | |||
===Filtration / affinity columns=== | |||
#[[Centrifugal filtration/Nucleic acids]] | #[[Centrifugal filtration/Nucleic acids]] | ||
#*for small DNA fragments (~50-200 bp in length) | #*for small DNA fragments (~50-200 bp in length) | ||
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#[[QIAquick PCR purification]] | #[[QIAquick PCR purification]] | ||
#*for typical DNA fragments (> 200 bp in length) | #*for typical DNA fragments (> 200 bp in length) | ||
[[Category:Protocol]] | [[Category:Protocol]] | ||
[[Category:DNA]] | [[Category:DNA]] | ||
[[Category:In vitro]] | [[Category:In vitro]] | ||
