Purification of DNA: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
grouped protocols according to method |
||
| Line 1: | Line 1: | ||
There are several methods for purifying DNA. The one you choose depends on the nature of your DNA sample and your downstream applications. | There are several methods for purifying DNA. The one you choose depends on the nature of your DNA sample and your downstream applications. | ||
===Alcohol precipitation=== | |||
#[[Nucleic acid precipitation]] | |||
#[[Ethanol precipitation of small DNA fragments]] | #[[Ethanol precipitation of small DNA fragments]] | ||
#[[Isopropanol Precipitation for PCR Purification]] | |||
#*use to concentrate DNA from PCR when you have verified that only a single PCR product exists. When performed properly this procedure exceeds the recovery of QIAquick PCR purification columns | |||
===Filtration / affinity columns=== | |||
#[[Centrifugal filtration/Nucleic acids]] | #[[Centrifugal filtration/Nucleic acids]] | ||
#*for small DNA fragments (~50-200 bp in length) | #*for small DNA fragments (~50-200 bp in length) | ||
| Line 9: | Line 15: | ||
#[[QIAquick PCR purification]] | #[[QIAquick PCR purification]] | ||
#*for typical DNA fragments (> 200 bp in length) | #*for typical DNA fragments (> 200 bp in length) | ||
[[Category:Protocol]] | [[Category:Protocol]] | ||
[[Category:DNA]] | [[Category:DNA]] | ||
[[Category:In vitro]] | [[Category:In vitro]] | ||
Revision as of 10:32, 25 April 2007
There are several methods for purifying DNA. The one you choose depends on the nature of your DNA sample and your downstream applications.
Alcohol precipitation
- Nucleic acid precipitation
- Ethanol precipitation of small DNA fragments
- Isopropanol Precipitation for PCR Purification
- use to concentrate DNA from PCR when you have verified that only a single PCR product exists. When performed properly this procedure exceeds the recovery of QIAquick PCR purification columns
Filtration / affinity columns
- Centrifugal filtration/Nucleic acids
- for small DNA fragments (~50-200 bp in length)
- removes proteins, nucleotides and salts
- Miniprep
- for purifying plasmid DNA from E. coli cells
- QIAquick PCR purification
- for typical DNA fragments (> 200 bp in length)
