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Griffin:Antibody Basics: Difference between revisions
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===Fab fragment=== | ===Fab fragment=== | ||
The 'F' stands for fragment and the 'ab' stands for antigen binding. This is the region where antigen binding takes place. The Fab portion of an antibody is made up of the N-terminal domains of both the heavy and light chains. This domain of the antibody is called the variable (V) domain (VH on the heavy chain, VL on the light chain) because there is such high variability between antibodies in this area. This allows recognition of thousands of different antigens. | The 'F' stands for fragment and the 'ab' stands for antigen binding. This is the region where antigen binding takes place. The Fab portion of an antibody is made up of the N-terminal domains of both the heavy and light chains. This domain of the antibody is called the variable (V) domain (VH on the heavy chain, VL on the light chain) because there is such high variability between antibodies in this area. This allows recognition of thousands of different antigens. | ||
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===HPLC Retention Times for Purified IgG=== | ===HPLC Retention Times for Purified IgG=== | ||
[[Image:PMID19911257.png|thumb|right| IgG Retention Times by HPLC]] | |||
*https://pubmed.ncbi.nlm.nih.gov/19911257/ | *https://pubmed.ncbi.nlm.nih.gov/19911257/ | ||
