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Griffin:Antibody Basics: Difference between revisions
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[[Image:antibody1.jpg|thumb|right|The basic structure of the heterotetrameric, disfulfide linked, Immunoglobulin gamma (IgG)]] | [[Image:antibody1.jpg|thumb|right|The basic structure of the heterotetrameric, disfulfide linked, Immunoglobulin gamma (IgG)]] | ||
[http://en.wikipedia.org/wiki/Antibody Antibodies] are B cell (lymphocyte) derived proteins produced by the immune system in order to address the immunological needs of a mammalian system over time. | [http://en.wikipedia.org/wiki/Antibody Antibodies] are B cell (lymphocyte) derived proteins produced by the immune system in order to address the immunological needs of a mammalian system over time. | ||
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===Fab fragment=== | ===Fab fragment=== | ||
[[Image:PMID19911257.png|thumb|right| IgG Retention Times by HPLC]] | |||
The 'F' stands for fragment and the 'ab' stands for antigen binding. This is the region where antigen binding takes place. The Fab portion of an antibody is made up of the N-terminal domains of both the heavy and light chains. This domain of the antibody is called the variable (V) domain (VH on the heavy chain, VL on the light chain) because there is such high variability between antibodies in this area. This allows recognition of thousands of different antigens. | The 'F' stands for fragment and the 'ab' stands for antigen binding. This is the region where antigen binding takes place. The Fab portion of an antibody is made up of the N-terminal domains of both the heavy and light chains. This domain of the antibody is called the variable (V) domain (VH on the heavy chain, VL on the light chain) because there is such high variability between antibodies in this area. This allows recognition of thousands of different antigens. | ||
