Griffin:Antibody Basics: Difference between revisions

Griffin:Antibody Basics (view source)

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 *1 constant (CL) (kappa or lambda) domain
 *1 variable (VL) (antigen Binding) domain
+===HPLC Retention Times for Purified IgG===
+*https://pubmed.ncbi.nlm.nih.gov/19911257/
+*HPLC retention times of monoclonal antibodies vs solubility (by ultrafiltration). Retention times are inversely related to solubility, with antibodies prone to precipitate at low concentrations in PBS being retained longer on the columns with broader peaks.
+*Retention Time @ ~10 peak is optimal solubility. candidates with low solubility and/or aggregate in PBS may indicate broader a peak retention time.
 ===F(ab')2 fragment===
@@ Line 72: / Line 78: @@
 *[https://www.thermofisher.com/antibody/product/Goat-anti-Mouse-IgM-Mu-chain-Secondary-Antibody-Polyclonal/16-5092-85 Goat Anti-Mouse IgM, µ chain]
 *[https://www.jacksonimmuno.com/catalog/products/115-095-020 Goat Anti-Mouse IgM, µ chain FITC]
-===HPLC Retention Times for Purified IgG===
-*https://pubmed.ncbi.nlm.nih.gov/19911257/
-*HPLC retention times of monoclonal antibodies vs solubility (by ultrafiltration). Retention times are inversely related to solubility, with antibodies prone to precipitate at low concentrations in PBS being retained longer on the columns with broader peaks.
-*Retention Time @ ~10 peak is optimal solubility. candidates with low solubility and/or aggregate in PBS may indicate broader a peak retention time.
 ==Primary antibody production==