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Griffin:Antibody Basics: Difference between revisions
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*https://pubmed.ncbi.nlm.nih.gov/19911257/ | *https://pubmed.ncbi.nlm.nih.gov/19911257/ | ||
*HPLC retention times of monoclonal antibodies vs solubility (by ultrafiltration). Retention times are inversely related to solubility, with antibodies prone to precipitate at low concentrations in PBS being retained longer on the columns with broader peaks. | *HPLC retention times of monoclonal antibodies vs solubility (by ultrafiltration). Retention times are inversely related to solubility, with antibodies prone to precipitate at low concentrations in PBS being retained longer on the columns with broader peaks. | ||
*Retention Time @ ~10 peak is optimal solubility. candidates with low solubility and/or aggregate in PBS may indicate broader a peak retention time. | |||
==Primary antibody production== | ==Primary antibody production== | ||
