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Griffin:Antibody Basics: Difference between revisions
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*[https://www.thermofisher.com/antibody/product/Goat-anti-Mouse-IgM-Mu-chain-Secondary-Antibody-Polyclonal/16-5092-85 Goat Anti-Mouse IgM, µ chain] | *[https://www.thermofisher.com/antibody/product/Goat-anti-Mouse-IgM-Mu-chain-Secondary-Antibody-Polyclonal/16-5092-85 Goat Anti-Mouse IgM, µ chain] | ||
*[https://www.jacksonimmuno.com/catalog/products/115-095-020 Goat Anti-Mouse IgM, µ chain FITC] | *[https://www.jacksonimmuno.com/catalog/products/115-095-020 Goat Anti-Mouse IgM, µ chain FITC] | ||
===HPLC Retention Times for Purified IgG=== | |||
[[Image:PMID19911257.png|thumb|right| IgG Retention Times by HPLC]] | |||
*https://pubmed.ncbi.nlm.nih.gov/19911257/ | |||
*HPLC retention times of monoclonal antibodies vs solubility (by ultrafiltration). Retention times are inversely related to solubility, with antibodies prone to precipitate at low concentrations in PBS being retained longer on the columns with broader peaks. | |||
==Primary antibody production== | ==Primary antibody production== | ||
