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Griffin:Antibody Basics: Difference between revisions
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F(ab')2 fragment secondary antibodies should be used when trying to avoid non-specific secondary antibody to Fc receptors on cell surfaces. | F(ab')2 fragment secondary antibodies should be used when trying to avoid non-specific secondary antibody to Fc receptors on cell surfaces. | ||
===[https://en.wikipedia.org/wiki/IGK@ Kappa Chain]=== | |||
*Immunoglobulin composition are disulphide linkage assembly of light chains and heavy chains. | |||
*Light chain (λ or κ) = ~220 amino acids, composed of variable domain, VL (~110 amino acids), and constant domain, CL (~110 amino acids). | |||
===[https://en.wikipedia.org/wiki/IGL@ Lambda Chain]=== | |||
*Immunoglobulin composition are disulphide linkage assembly of light chains and heavy chains. | |||
*Light chain (λ or κ) = ~220 amino acids, composed of variable domain, VL (~110 amino acids), and constant domain, CL (~110 amino acids). | |||
===[https://en.wikipedia.org/wiki/Immunoglobulin_M Mu Chain]=== | |||
*Early humoral response pentameric IgM (macro) | |||
*~576 amino acids IgM µ heavy chain = ~110 aa variable domain (VH) + 4~110 aa constant regions (Cµ1, Cµ2, Cµ3, Cµ4) + ~20aa tail | |||
*light chain (L) + heavy chain(µ) = 2x (Cµ2 disulfide linked) µL units =(µL)2 = IgM “monomer”= ~IgG. | |||
*[https://www.thermofisher.com/antibody/product/Goat-anti-Mouse-IgM-Mu-chain-Secondary-Antibody-Polyclonal/16-5092-85 Goat-anti-Mouse-IgM-Mu] | |||
*[https://www.jacksonimmuno.com/catalog/products/115-095-020 Goat Anti-Mouse IgM, µ chain FITC] | |||
==Primary antibody production== | ==Primary antibody production== | ||
