E. coli genotypes: Difference between revisions

*See the original Studier [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3537305&query_hl=14 paper] or the summary in [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2199796&query_hl=13&itool=pubmed_docsum Methods in Enzymology] for more details.

*Whole genome sequence available [http://www.ncbi.nlm.nih.gov/genomes/lproks.cgi]

===BL21(DE3) pLysS===  

* The pLysS plasmid encodes T7 phage lysozyme, an inhibitor for T7 polymerase which reduces and almost eliminates expression from transformed T7 promoter containing plasmids when not induced.

* see Moffatt87 for details of pLysS and pLysE plasmids

===BLR===

===BW313===

Hfr ung-1 relA1 dut-1 spoT1 thi-1

<biblio>

#Kunkel-PNAS-1985 pmid=3881765

*streptomycin resistant

*appears to NOT contain lacI (based on a colony PCR) --[[User:Austin J. Che|Austin Che]] 16:16, 18 June 2007 (EDT)

<biblio>

#Bernard-JMolBiol-1992 pmid=1324324

* The strain is meant as a universal Staphylococcal cloning host

* Streptomycin resistant

<biblio>

#Monk-MBio-2012 pmid=22434850

endA1 hsdR17 glnV44 (= supE44) thi-1 recA1 gyrA96 relA1 φ80dlacΔ(lacZ)M15 Δ(lacZYA-argF)U169 zdg-232::Tn10 uidA::pir+

* tetracyclin resistant

<biblio>

#Platt-Plasmid-1990 pmid=10610816

zdg-232 uidA::pir116

* tetracyclin resistant

<biblio>

#Platt-Plasmid-1990 pmid=10610816

Hfr Su+ supF

* phosphatase positive strain with H12 amber mutation in the phosphatase gene

<biblio>

#Garen-JMolBiol-1965 pmid=5327650

* Plasmids isolated from this E. coli strain transform S. aureus clonal complex 1 - MW2 at high efficiency

* Streptomycin resistant

<biblio>

#Monk-MBio-2015 pmid=26015493

* Plasmids isolated from this E. coli strain transform S. aureus clonal complex 8 - NRS384 at high efficiency

* Streptomycin resistant

<biblio>

#Monk-MBio-2015 pmid=26015493

* Plasmids isolated from this E. coli strain transform S. aureus clonal complex 30 - MRSA252 at high efficiency

* Streptomycin resistant

<biblio>

#Monk-MBio-2015 pmid=26015493

* Plasmids isolated from this E. coli strain transform S. aureus sequence type 93-JKD6159 at high efficiency

* Streptomycin resistant

<biblio>

#Monk-MBio-2015 pmid=26015493

''hfrH'' ''relA1'' ''spoT1'' ''thi-1''

* This strain is designed for propagation of F+ specific phages

<biblio>

#Loomis-JMolBiol-1967 pmid=5340246

* The resident λ is a defective lysogen. .

* cI857 is ind-

<biblio>

#Bernard-Gene-1979 pmid=372049

* The resident λ is a defective lysogen.

* cI857 is ind-

<biblio>

#Remaut-Gene-1981 pmid=6271633

*References:

**[http://cgsc2.biology.yale.edu/Strain.php?ID=11225 E. coli Genetic Stock Center, MC1061 Record]

**Complete DH10B sequence is available, see Durfee et al., 2008.

<biblio>

* r_k^–m_k⁺

* recommended growth at 28°C

<biblio>

#Mertens-Gene-1995 pmid=7590329

* rifampicin resistant

* r_k^–m_k⁺

<biblio>

#Casadaban-JMolBio-1980 pmid=6997493

* Kanamycin resistant

<biblio>

#Mika-FaradayDiscuss-2015 pmid=26449690

* Kanamycin resistant

<biblio>

#Mika-FaradayDiscuss-2015 pmid=26449690

* Kanamycin resistant

<biblio>

#Mika-FaradayDiscuss-2015 pmid=26449690

Hfr(PO2A) fhuA22 garB10 ompF627 fadL701 relA1 pit10 spoT1 metB1 mcrB1 cre-510 phoA4

* This is a phosphatase negative strain, carrying the S26 amber mutation (TAG) in the phosphatase gene

<biblio>

#Garen-JMolBiol-1965 pmid=5327650

* This is a phosphatase negative strain, carrying the S26 amber mutation (TAG) in the phosphatase gene

* The strain carries the supE suppressor (insers glutamine at the amber position) and is therefore capable of suppressing the S26 phosphatase nonsense mutation, as well as amber mutations in other genes. However, the efficiency of suppression may vary considerably depending on the sequence context of the amber codon.

<biblio>

#Garen-JMolBiol-1965 pmid=5327650

* This is a phosphatase negative strain, carrying the S26 amber mutation (TAG) in the phosphatase gene

* The strain carries the supD suppressor (insers serine at the amber position) and is therefore capable of suppressing the S26 phosphatase nonsense mutation, as well as amber mutations in other genes. However, the efficiency of suppression may vary considerably depending on the sequence context of the amber codon.

<biblio>

#Garen-JMolBiol-1965 pmid=5327650

===SG4121===

lon-100 Δ(gal-pgl)324 lac thi rpsL recA  hsdR  

<biblio>

#Keidel-EurJBiochem-1992 pmid=1312934

===SM10(λpir)===

thi thr leu tonA lacY supE recA::RP4-2-Tc::Mu Km λpir

<biblio>

#Simon-BioTech-1983

* Useful strain for site-directed mutagenesis: isabled mismatch repair due to knocked-out mutS

* Not to be used as permanent storage host for plasmids because of the danger of accumulating spontaneous mutations.

<biblio>

#Zell-EMBOJ-1987 pmid=3038536