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=='''Protocol'''== | =='''Protocol'''== | ||
For 1 L LFM | For 1 L LFM | ||
*Mix 1.7 g LFM powder + 5 g ammonium sulfate + water to 900 mL and autoclave | |||
*Once cooled to ~55 ° C, add 50 mL of 20x glucose solution and 50 mL of appropriate 20x amino acid mix. | |||
LFM powder = YNB w/o ammonium sulfate, w/o folic acid, w/o riboflavin | |||
==Notes== | ==Notes== |
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