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McClean:LFM Recipe: Difference between revisions
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=='''Protocol'''== | =='''Protocol'''== | ||
For 1 L LFM | For 1 L LFM | ||
1. Mix 1.7 g LFM powder* + 5 g ammonium sulfate + water to 900 mL and autoclave | 1.Mix 1.7 g LFM powder* + 5 g ammonium sulfate + water to 900 mL and autoclave | ||
2. Once cooled to ~55 ° C, add 50 mL of 20x glucose solution and 50 mL of appropriate 20x amino acid mix. | 2.Once cooled to ~55 ° C, add 50 mL of 20x glucose solution and 50 mL of appropriate 20x amino acid mix. | ||
*LFM powder = YNB w/o ammonium sulfate, w/o folic acid, w/o riboflavin | *LFM powder = YNB w/o ammonium sulfate, w/o folic acid, w/o riboflavin | ||
==Notes== | ==Notes== | ||
