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User:Miroslav Gasparek: Difference between revisions
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One of the factors determining the success of the effort to build an artificial cell is our ability to characterize and understand the function of the genes essential for the viability of life. The smallest viable living organism, JCVI-syn3.0 contains 473 genes, of which 149 is of unknown function. My specific research goal is to facilitate our understanding of the function of these essential (and quasi-essential) genes for life through the experimental development of the protein-protein interaction network of the JCVI-syn3.0 and computational investigation of the properties of the unknown essential genes. </p> | One of the factors determining the success of the effort to build an artificial cell is our ability to characterize and understand the function of the genes essential for the viability of life. The smallest viable living organism, JCVI-syn3.0 contains 473 genes, of which 149 is of unknown function. My specific research goal is to facilitate our understanding of the function of these essential (and quasi-essential) genes for life through the experimental development of the protein-protein interaction network of the JCVI-syn3.0 and computational investigation of the properties of the unknown essential genes. </p> | ||
<p> | <p> | ||
''Update (December 3 2018)'' | '''Update (December 3 2018): Final Report''' | ||
In the end, after a lot of work, failures, lessons learned, and sleepless nights... we got some results! The detailed report of my experimental work on the modification of the bait and prey plasmids for the protein-fragment complementation assay can be found here:[[Media:29Nov2018_StanfordReport_Final_MG.pdf | Changing | In the end, after a lot of work, failures, lessons learned, and sleepless nights... we got some results! The detailed report of my experimental work on the modification of the bait and prey plasmids for the protein-fragment complementation assay can be found here:[[Media:29Nov2018_StanfordReport_Final_MG.pdf | Changing | ||
the cloning compatibility of the plasmid vectors for the high-throughput | the cloning compatibility of the plasmid vectors for the high-throughput | ||
