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BioMicroCenter:Microarrays: Difference between revisions
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The MIT BioMicro Center retains minimal support for Agilent and Affymetrix Microarrays. Assisted microarray serves are no longer available. The Agilent oven and microarray scanner are available to users who prepare their own arrays and remains under service contract. Our Affymetrix instrumentation is available for use at user's risk. | The MIT BioMicro Center retains minimal support for Agilent and Affymetrix Microarrays. Assisted microarray serves are no longer available. The Agilent oven and microarray scanner are available to users who prepare their own arrays and remains under service contract. Our Affymetrix instrumentation is available for use at user's risk. | ||
Please note that the BioMicro Center no longer supports printing of microarrays in house. Custom oligonuclotide arrays are available from Agilent at the same cost as their commercial arrays using their [http://www.chem.agilent.com/en-US/products/instruments/dnamicroarrays/pages/gp50660.aspx eArray system] (we can [[BioMicroCenter:Computing#BioInformatics_Services|help you]] through the process of designing arrays). For anyone requiring microarray printing, we encourage you to contact [http://jura.wi.mit.edu/genomecorewiki/index.php/Tom_Volkert Tom Volkert at the Whitehead Institute CGT]. | Please note that the BioMicro Center no longer supports printing of microarrays in house. Custom oligonuclotide arrays are available from Agilent at the same cost as their commercial arrays using their [http://www.chem.agilent.com/en-US/products/instruments/dnamicroarrays/pages/gp50660.aspx eArray system] (we can [[BioMicroCenter:Computing#BioInformatics_Services|help you]] through the process of designing arrays). For anyone requiring microarray printing, we encourage you to contact [http://jura.wi.mit.edu/genomecorewiki/index.php/Tom_Volkert Tom Volkert at the Whitehead Institute CGT]. | ||
=== | == MICROARRAY SCANNER == | ||
Agilent | {| align=left | ||
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! Instrument || Agilent DNA scanner | |||
|- | |||
| TYPE || WALKUP - MIT only | |||
|- | |||
| UNIT || Per Slide | |||
|- | |||
| FORMAT || Glass slides | |||
|- | |||
| SIGNUP || [https://mit.ilabsolutions.com/equipment/show/261696/?tab=schedule ilabs] | |||
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| NEW USERS || New users should request training by emailing biomicro@mit.edu | |||
|- | |||
| DONATED BY || Dr. Stephen Bell. <BR> Upgrade by Dept of Biology. | |||
|} | |||
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The Agilent scanner provides red/green scanning of glass slides at 2μm resolution. While built for Agilent arrays, the scanner is capable of handling a broad spectrum of different printed arrays. Agilent Feature Extractor provides basic data analysis as well as quality control. We are currently running Feature extractor version 10.5. Gasket slides and ozone protection covers designed for Agilent arrays are available in the lab. | |||
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