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Griffin:siRNA transfection: Difference between revisions
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Griffin:siRNA transfection (view source)
Revision as of 14:40, 26 September 2018
, 26 September 2018→siRNA calculation and reconstitution
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Chemically synthesized 21-25 nucleotide siRNA are suitable for [http://en.wikipedia.org/wiki/Transfection transfection] into dividing cells. A standard siRNA molecule is a chemically synthesized, 19 base pair double stranded RNA molecule with 2 base pair overhangs on the 3' ends (total of 21 base pair). Calculating the molecular weight of a single base pair based on chemical formula (at pH 7) gives ~615.4 Daltons/base pair on average+2 Na+ gives ~46 extra Daltons per base pair, or 661.4 Da/base pair total (average of GC & AT). | Chemically synthesized 21-25 nucleotide siRNA are suitable for [http://en.wikipedia.org/wiki/Transfection transfection] into dividing cells. A standard siRNA molecule is a chemically synthesized, 19 base pair double stranded RNA molecule with 2 base pair overhangs on the 3' ends (total of 21 base pair). Calculating the molecular weight of a single base pair based on chemical formula (at pH 7) gives ~615.4 Daltons/base pair on average+2 Na+ gives ~46 extra Daltons per base pair, or 661.4 Da/base pair total (average of GC & AT). | ||
*Molarity of siRNA vialed: 10 uM ( uM/L ) | *Molarity of siRNA vialed: 10 uM ( uM/L ) === 10 picomol / uL | ||
*Volume after reconstitution: 330 uL | *Volume after reconstitution: 330 uL | ||
