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Griffin:Antibody Basics: Difference between revisions
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The 'F' stands for fragment and the 'ab' stands for antigen binding. This is the region where antigen binding takes place. The Fab portion of an antibody is made up of the N-terminal domains of both the heavy and light chains. This domain of the antibody is called the variable (V) domain (VH on the heavy chain, VL on the light chain) because there is such high variability between antibodies in this area. This allows recognition of thousands of different antigens. | The 'F' stands for fragment and the 'ab' stands for antigen binding. This is the region where antigen binding takes place. The Fab portion of an antibody is made up of the N-terminal domains of both the heavy and light chains. This domain of the antibody is called the variable (V) domain (VH on the heavy chain, VL on the light chain) because there is such high variability between antibodies in this area. This allows recognition of thousands of different antigens. | ||
Fab fragment antibodies are generated through papain digestion of whole IgG to remove entire Fc portion, including the hinge region. Fab are monovalent, containing only a single antigen binding site. molecular weight Fab fragments ~50 kDa. | |||
====Kappa/Lambda Ratios==== | ====Kappa/Lambda Ratios==== | ||
