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• 09:46, 16 January 2023 JCAnderson talk contribs created page JCAOligoTutorial2b prompt (Created page with "<pre>I am trying to understand a tutorial about the recombinant DNA technique SOEing. The technique is used to silently remove a restriction site when cloning a gene to make a BioBrick part. The experiment involves design of 2 oligos to PCR the region up to the restriction site, and then 2 oligos to amplify the region after the site. Those two PCRs are joined in a SOEing reaction to make a new PCR product without the restriction site. This is then then digested with...")
• 09:31, 16 January 2023 JCAnderson talk contribs created page JCAOligoTutorial1b prompt (Created page with "<code>I am trying to learn recombinant DNA technology. The experiment involves designing 2 oligonucleotides composed of a 5 bp 5' tail of arbitrary sequence, a restriction site polylinker, and the annealing region to amplify a template sequence. I then need to document my experiment in this format: "PCR ca1067F/ca1067R on pSB1AK3-b0015 (1054bp, pcrpdt) Digest pcrpdt (EcoRI/SpeI, 10+1038+6, 1, pcrdig) Digest pSB1A2-I13521 (EcoRI...")
• 08:40, 30 January 2020 JCAnderson talk contribs created page Anderson GoldenGateTutorialSolution (Created page with "//Replace the SmR gene in pTargetF by Bla pcr yyBla-F/yyBla-R on p20N5 (1183 bp, fragA) pcr yyEI-F/yyEI-R on pTargetF (1174 bp, fragB) assemble fragA, fragB (BsaI, pTarg1) Tr...")
• 08:26, 14 September 2019 JCAnderson talk contribs uploaded File:JCAseq GGExample.zip
• 08:26, 14 September 2019 JCAnderson talk contribs created page File:JCAseq GGExample.zip
• 22:12, 11 September 2019 JCAnderson talk contribs created page Arking:JCAOligoTutoriaGG (Created page with "This tutorial describes the process of designing oligos for performing Golden Gate assembly reactions. Golden Gate cloning and related methods use a typeIIs restriction endon...")
• 21:14, 1 April 2019 JCAnderson talk contribs created page Synthetic Biology Trails (Created page with "These videos provide an overview of synthetic biology background, methods, and design ====Lectures on experimental design==== The DNA Manipulation Enzymes<br> https://www.you...")
• 18:52, 31 January 2019 JCAnderson talk contribs uploaded File:Genbank hint.png
• 18:52, 31 January 2019 JCAnderson talk contribs created page File:Genbank hint.png
• 18:13, 22 January 2019 JCAnderson talk contribs uploaded File:Basic Molecular Biology.pdf
• 18:13, 22 January 2019 JCAnderson talk contribs created page File:Basic Molecular Biology.pdf
• 16:56, 5 September 2017 JCAnderson talk contribs uploaded File:Bare Bones Biochemistry.pdf
• 11:09, 6 February 2014 JCAnderson talk contribs uploaded File:Proximity ligation.pdf
• 11:07, 6 February 2014 JCAnderson talk contribs uploaded File:JCA test puppy.jpg
• 08:23, 13 March 2013 JCAnderson talk contribs uploaded File:2013 03 13-JCA-gel1.jpg
• 22:58, 12 February 2013 JCAnderson talk contribs uploaded File:UCBerk2007iGEM - Sunday.pptx
• 12:17, 12 February 2013 JCAnderson talk contribs uploaded File:Example5.jpg
• 11:23, 7 February 2012 JCAnderson talk contribs uploaded File:Sbb12 spencer scott.png
• 08:58, 7 February 2012 JCAnderson talk contribs uploaded File:PBca9525-Bjc0005.str
• 20:00, 6 February 2012 JCAnderson talk contribs uploaded File:Sbb12 ci857.str
• 19:15, 6 February 2012 JCAnderson talk contribs uploaded File:PKGC438.str
• 19:02, 6 February 2012 JCAnderson talk contribs uploaded File:PBgl00001-Brp0006.str
• 18:42, 6 February 2012 JCAnderson talk contribs uploaded File:PBgl00001-Bjk2828.str
• 18:27, 6 February 2012 JCAnderson talk contribs uploaded File:PBjk2741-jtk2801.str
• 18:27, 6 February 2012 JCAnderson talk contribs uploaded File:PBgl00001-Bca1859.str
• 17:18, 6 February 2012 JCAnderson talk contribs uploaded File:PBca9525-sbb1230.str
• 21:02, 5 February 2012 JCAnderson talk contribs uploaded File:PBca9525-bgl1031.str
• 19:16, 5 February 2012 JCAnderson talk contribs uploaded File:PBca9525-Bca1835.str
• 19:12, 5 February 2012 JCAnderson talk contribs uploaded File:PBca9525-Bca1832.str
• 19:04, 5 February 2012 JCAnderson talk contribs uploaded File:PBca9145-jtk2768.str
• 18:20, 5 February 2012 JCAnderson talk contribs uploaded File:PBjh1601CK-Bjh2128.str
• 18:10, 5 February 2012 JCAnderson talk contribs uploaded File:PBjk2741-jtk3346.str
• 10:32, 5 February 2012 JCAnderson talk contribs uploaded File:PBAD-T4L.str
• 09:53, 5 February 2012 JCAnderson talk contribs uploaded File:PBca9525-Bca1839.str
• 09:53, 5 February 2012 JCAnderson talk contribs uploaded File:PBca9525-Bca1834.str
• 09:51, 5 February 2012 JCAnderson talk contribs uploaded File:Media-pBca9525-Bca1834.str
• 09:50, 5 February 2012 JCAnderson talk contribs uploaded File:Media-pBca9525-Bca1834.seq
• 09:49, 5 February 2012 JCAnderson talk contribs uploaded File:Sbb2012 homodimerParts.pdf
• 20:47, 31 January 2012 JCAnderson talk contribs uploaded File:SBB12logo.png
• 18:04, 24 January 2012 JCAnderson talk contribs uploaded File:SelfTm.png
• 12:47, 10 March 2011 JCAnderson talk contribs uploaded File:031011-HelenGel.png
• 12:32, 9 March 2011 JCAnderson talk contribs uploaded File:030811-mysterygel3.png
• 12:30, 9 March 2011 JCAnderson talk contribs uploaded File:030811-mysterygel2.png
• 18:58, 8 March 2011 JCAnderson talk contribs uploaded File:030811-mysterygel1.png
• 18:55, 8 March 2011 JCAnderson talk contribs uploaded File:030811-xinxingel.png
• 17:55, 21 February 2011 JCAnderson talk contribs uploaded File:022111-AnalGel3.jpg
• 17:53, 21 February 2011 JCAnderson talk contribs uploaded File:022111-AnalGel4.jpg
• 17:34, 21 February 2011 JCAnderson talk contribs uploaded File:022111-AnalGel2.jpg
• 17:32, 21 February 2011 JCAnderson talk contribs uploaded File:022111-AnalGel1.jpg
• 14:35, 18 February 2011 JCAnderson talk contribs uploaded File:021811-AnalGel2.jpg