Uploads by David M. Gray

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This special page shows all uploaded files.

File list
Date Name Thumbnail Size Description
03:53, 21 August 2013 Session4.png (file) 29 KB Shows where we cut the components of our vector with restriction enzymes.
03:48, 21 August 2013 Session 4.svg (file) 6 KB Illustration showing the cuts in our vector made with various restriction enzymes.
01:44, 9 August 2013 Overlapping Segments.png (file) 3 KB This diagram shows how we assemble the emGFP coding sequence from shorter strands of DNA. Adjacent segments are from complimentary strands and designed to overlap such that their nucleotides will have a unique matching set. The gaps are filled in with n
00:06, 9 August 2013 Vector Construction.jpg (file) 33 KB The plan of the plasmid we will be constructing consists of three segments we will be splicing together. The promoter + RBS, the main part of the vector including the terminator and the emGFP coding section.