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Jump to navigationJump to search- 07:40, 27 October 2010 (diff | hist) (-20) IGEM:University of Debrecen: transfection (current)
- 07:39, 27 October 2010 (diff | hist) (-2) IGEM:University of Debrecen: transfection
- 07:37, 27 October 2010 (diff | hist) (-6) IGEM:University of Debrecen: transfection
- 07:36, 27 October 2010 (diff | hist) (-9) IGEM:University of Debrecen: transfection
- 07:35, 27 October 2010 (diff | hist) (+2) IGEM:University of Debrecen: transfection
- 07:35, 27 October 2010 (diff | hist) (-1) IGEM:University of Debrecen: transfection
- 13:00, 26 October 2010 (diff | hist) (+2) IGEM:University of Debrecen: Polymerase Chain Reaction (PCR) (→An example program) (current)
- 12:48, 26 October 2010 (diff | hist) (+8) IGEM:University of Debrecen: Polymerase Chain Reaction (PCR)
- 12:47, 26 October 2010 (diff | hist) (+7) IGEM:University of Debrecen: Polymerase Chain Reaction (PCR)
- 12:44, 26 October 2010 (diff | hist) (+2,368) N IGEM:University of Debrecen: Polymerase Chain Reaction (PCR) (New page: ==Overview== PCR is an acronym for polymerase chain reaction. It is a method for amplifying DNA in vitro. ==Reagents== • Expand High Fidelity Buffer with MgCl2 • PCR grade nucleotide...)
- 08:50, 25 August 2010 (diff | hist) (+5,456) N Editing IGEM:University of Debrecen: Mini Prep (New page: ==Scientific Background== This protocol is designed for purification of up to 20 μg of high-copy plasmid DNA from 1–5 ml overnight cultures of E. coli in LB (Luria-Bertani) medium. =...)
- 08:34, 25 August 2010 (diff | hist) (+8) N IGEM:University of Debrecen: Measurements with Victor machine (New page: deleteme) (current)
- 07:47, 25 August 2010 (diff | hist) (-328) IGEM:University of Debrecen:Restriction biobrick parts
- 07:35, 25 August 2010 (diff | hist) (+5,427) IGEM:University of Debrecen:Restriction biobrick parts (→Procedure)
- 07:16, 25 August 2010 (diff | hist) (-1) IGEM:University of Debrecen:Restriction biobrick parts
- 07:16, 25 August 2010 (diff | hist) (-19,550) IGEM:University of Debrecen:Restriction biobrick parts
- 06:57, 25 August 2010 (diff | hist) (+2,369) N IGEM:University of Debrecen: Enzymatic activity with Victor machine (New page: ==Scientific Background== The Wallac 1420 VICTOR2 is a multilabel, multitask plate reader designed to support the future demands of industrial and academic laboratories for multiple assay ...)
- 05:56, 25 August 2010 (diff | hist) (-3,112) IGEM:University of Debrecen:Midi Prep (Replacing page with 'deleteme') (current)
- 05:54, 25 August 2010 (diff | hist) (+4) IGEM:University of Debrecen: Midi Prep
- 03:27, 25 August 2010 (diff | hist) (+3,120) N IGEM:University of Debrecen:Midi Prep (New page: ==Scientific Background== ==Overview== ==Materials== ==Procedure== 1. Centrifuge bacterial cells from 30 ml culture grown in LB medium by centrifuging for 10 min at 5000 rpm, 4°C Dis...)
- 07:26, 24 August 2010 (diff | hist) (+46) IGEM:University of Debrecen: Midi Prep
- 07:24, 24 August 2010 (diff | hist) (-151) IGEM:University of Debrecen: Midi Prep
- 06:53, 24 August 2010 (diff | hist) (+4,687) IGEM:University of Debrecen: Midi Prep
- 01:59, 24 August 2010 (diff | hist) (+119) N IGEM:University of Debrecen: Midi Prep (New page: ==Scientific Background== ==Overview== ==Materials== ==Procedure== ==Notes & troubleshooting== ==References== ==Other==)
- 01:56, 24 August 2010 (diff | hist) (0) IGEM:University of Debrecen:Transformation of competent cells (→Procedure)
- 01:55, 24 August 2010 (diff | hist) (+1,755) N IGEM:University of Debrecen:Transformation of competent cells (New page: ==Scientific Background== Transformation is the process of introducing foreign DNA (e.g plasmids, BAC) into a bacterium. Bacterial cells into which foreign DNA can be transformed are call...)
- 07:42, 23 August 2010 (diff | hist) (+159) IGEM:University of Debrecen: Transfection (→Procedure)
- 07:36, 23 August 2010 (diff | hist) (+1,051) IGEM:University of Debrecen: Transfection
- 07:21, 23 August 2010 (diff | hist) (+3,601) N IGEM:University of Debrecen: Transfection (New page: Transfection protocol with PEI nuclear receptor construct (for COS1 cells) ==Scientific Background== The following protocol is used to succesfully introduce foreign plasmid DNA into plur...)
- 05:41, 23 August 2010 (diff | hist) (+3,158) N IGEM:University of Debrecen: Media PEI Preparation (New page: Preparing medias for eukaryotic cell culturing, preparing PEI solution for transfection ==Scientific Background== I. The Media Eukaryotic cells, derived from multicellular animal eukaryo...)
- 04:52, 23 August 2010 (diff | hist) (+6,764) N IGEM:University of Debrecen:Cell Passaging (New page: ==Scientific Background== Medium reconstitution: You have to reconstitute a new bottle of medium when you don't have enough medium for the subsequent processes (cell culturing, Transfecti...)
- 02:20, 23 August 2010 (diff | hist) (+1,565) N IGEM:University of Debrecen:PCR Purification from Solution (New page: PCR products purification from Solution: ==Scientific Background== ==Overview== ==Materials== Centrifuge Vortex pipett (100-1000µl and 2-20µl) tips (blue and yellow) ice a...)
- 01:54, 23 August 2010 (diff | hist) (+14) IGEM:University of Debrecen:PCR purification from Agarose Gel
- 01:53, 23 August 2010 (diff | hist) (+2,113) N IGEM:University of Debrecen:PCR purification from Agarose Gel (New page: DNA Fragments Purification from Agarose Gel ==Scientific Background== ==Overview== ==Materials== Centrifuge Vortex Incubator Pipet (100-1000µl and 2-20µl) Tips (blue and yellow) Ice a...)
- 04:18, 15 August 2010 (diff | hist) (+1,012) N IGEM:University of Debrecen:Gel electrophoresis (New page: Gel Electrophoresis: ==Scientific Background== ==Overview== ==Materials== ==Procedure== 1. masure 1 g agarose (for 1% gel agarose) 2. put it into a sterilized bottle 3. masure 100 ml...)
- 03:54, 15 August 2010 (diff | hist) (-939) IGEM:University of Debrecen:Ligand Treatment
- 05:42, 10 August 2010 (diff | hist) (+6,634) N IGEM:University of Debrecen:Ligand Treatment (New page: ==Scientific Background== We use ligand treatment in order to activate the transfected nuclear receptors in eukaryote systems (COS1 cells). After ligand binding the nuclear receptors bind...)
- 12:06, 13 July 2010 (diff | hist) (+1,138) N User:Yakir Guri (current)
- 12:06, 13 July 2010 (diff | hist) (+3,182) N User talk:Yakir Guri (current)
