User contributions for Yakir Guri

27 October 2010

• 14:4014:40, 27 October 2010 diff hist −20 IGEM:University of Debrecen: transfection No edit summary current
• 14:3914:39, 27 October 2010 diff hist −2 IGEM:University of Debrecen: transfection No edit summary
• 14:3714:37, 27 October 2010 diff hist −6 IGEM:University of Debrecen: transfection No edit summary
• 14:3614:36, 27 October 2010 diff hist −9 IGEM:University of Debrecen: transfection No edit summary
• 14:3514:35, 27 October 2010 diff hist +2 IGEM:University of Debrecen: transfection No edit summary
• 14:3514:35, 27 October 2010 diff hist −1 IGEM:University of Debrecen: transfection No edit summary

26 October 2010

• 20:0020:00, 26 October 2010 diff hist +2 IGEM:University of Debrecen: Polymerase Chain Reaction (PCR) →An example program current
• 19:4819:48, 26 October 2010 diff hist +8 IGEM:University of Debrecen: Polymerase Chain Reaction (PCR) No edit summary
• 19:4719:47, 26 October 2010 diff hist +7 IGEM:University of Debrecen: Polymerase Chain Reaction (PCR) No edit summary
• 19:4419:44, 26 October 2010 diff hist +2,368 N IGEM:University of Debrecen: Polymerase Chain Reaction (PCR) New page: ==Overview== PCR is an acronym for polymerase chain reaction. It is a method for amplifying DNA in vitro. ==Reagents== • Expand High Fidelity Buffer with MgCl2 • PCR grade nucleotide...

25 August 2010

• 15:5015:50, 25 August 2010 diff hist +5,456 N Editing IGEM:University of Debrecen: Mini Prep New page: ==Scientific Background== This protocol is designed for purification of up to 20 μg of high-copy plasmid DNA from 1–5 ml overnight cultures of E. coli in LB (Luria-Bertani) medium. =...
• 15:3415:34, 25 August 2010 diff hist +8 N IGEM:University of Debrecen: Measurements with Victor machine New page: deleteme current
• 14:4714:47, 25 August 2010 diff hist −328 IGEM:University of Debrecen:Restriction biobrick parts No edit summary
• 14:3514:35, 25 August 2010 diff hist +5,427 IGEM:University of Debrecen:Restriction biobrick parts →Procedure
• 14:1614:16, 25 August 2010 diff hist −1 IGEM:University of Debrecen:Restriction biobrick parts No edit summary
• 14:1614:16, 25 August 2010 diff hist −19,550 IGEM:University of Debrecen:Restriction biobrick parts No edit summary
• 13:5713:57, 25 August 2010 diff hist +2,369 N IGEM:University of Debrecen: Enzymatic activity with Victor machine New page: ==Scientific Background== The Wallac 1420 VICTOR2 is a multilabel, multitask plate reader designed to support the future demands of industrial and academic laboratories for multiple assay ...
• 12:5612:56, 25 August 2010 diff hist −3,112 IGEM:University of Debrecen:Midi Prep Replacing page with 'deleteme' current
• 12:5412:54, 25 August 2010 diff hist +4 IGEM:University of Debrecen: Midi Prep No edit summary
• 10:2710:27, 25 August 2010 diff hist +3,120 N IGEM:University of Debrecen:Midi Prep New page: ==Scientific Background== ==Overview== ==Materials== ==Procedure== 1. Centrifuge bacterial cells from 30 ml culture grown in LB medium by centrifuging for 10 min at 5000 rpm, 4°C Dis...

24 August 2010

• 14:2614:26, 24 August 2010 diff hist +46 IGEM:University of Debrecen: Midi Prep No edit summary
• 14:2414:24, 24 August 2010 diff hist −151 IGEM:University of Debrecen: Midi Prep No edit summary
• 13:5313:53, 24 August 2010 diff hist +4,687 IGEM:University of Debrecen: Midi Prep No edit summary
• 08:5908:59, 24 August 2010 diff hist +119 N IGEM:University of Debrecen: Midi Prep New page: ==Scientific Background== ==Overview== ==Materials== ==Procedure== ==Notes & troubleshooting== ==References== ==Other==
• 08:5608:56, 24 August 2010 diff hist 0 IGEM:University of Debrecen:Transformation of competent cells →Procedure
• 08:5508:55, 24 August 2010 diff hist +1,755 N IGEM:University of Debrecen:Transformation of competent cells New page: ==Scientific Background== Transformation is the process of introducing foreign DNA (e.g plasmids, BAC) into a bacterium. Bacterial cells into which foreign DNA can be transformed are call...

23 August 2010

• 14:4214:42, 23 August 2010 diff hist +159 IGEM:University of Debrecen: Transfection →Procedure
• 14:3614:36, 23 August 2010 diff hist +1,051 IGEM:University of Debrecen: Transfection No edit summary
• 14:2114:21, 23 August 2010 diff hist +3,601 N IGEM:University of Debrecen: Transfection New page: Transfection protocol with PEI nuclear receptor construct (for COS1 cells) ==Scientific Background== The following protocol is used to succesfully introduce foreign plasmid DNA into plur...
• 12:4112:41, 23 August 2010 diff hist +3,158 N IGEM:University of Debrecen: Media PEI Preparation New page: Preparing medias for eukaryotic cell culturing, preparing PEI solution for transfection ==Scientific Background== I. The Media Eukaryotic cells, derived from multicellular animal eukaryo...
• 11:5211:52, 23 August 2010 diff hist +6,764 N IGEM:University of Debrecen:Cell Passaging New page: ==Scientific Background== Medium reconstitution: You have to reconstitute a new bottle of medium when you don't have enough medium for the subsequent processes (cell culturing, Transfecti...
• 09:2009:20, 23 August 2010 diff hist +1,565 N IGEM:University of Debrecen:PCR Purification from Solution New page: PCR products purification from Solution: ==Scientific Background== ==Overview== ==Materials== Centrifuge Vortex pipett (100-1000µl and 2-20µl) tips (blue and yellow) ice a...
• 08:5408:54, 23 August 2010 diff hist +14 IGEM:University of Debrecen:PCR purification from Agarose Gel No edit summary
• 08:5308:53, 23 August 2010 diff hist +2,113 N IGEM:University of Debrecen:PCR purification from Agarose Gel New page: DNA Fragments Purification from Agarose Gel ==Scientific Background== ==Overview== ==Materials== Centrifuge Vortex Incubator Pipet (100-1000µl and 2-20µl) Tips (blue and yellow) Ice a...

15 August 2010

• 11:1811:18, 15 August 2010 diff hist +1,012 N IGEM:University of Debrecen:Gel electrophoresis New page: Gel Electrophoresis: ==Scientific Background== ==Overview== ==Materials== ==Procedure== 1. masure 1 g agarose (for 1% gel agarose) 2. put it into a sterilized bottle 3. masure 100 ml...
• 10:5410:54, 15 August 2010 diff hist −939 IGEM:University of Debrecen:Ligand Treatment No edit summary

10 August 2010

• 12:4212:42, 10 August 2010 diff hist +6,634 N IGEM:University of Debrecen:Ligand Treatment New page: ==Scientific Background== We use ligand treatment in order to activate the transfected nuclear receptors in eukaryote systems (COS1 cells). After ligand binding the nuclear receptors bind...

13 July 2010

• 19:0619:06, 13 July 2010 diff hist +3,182 N User talk:Yakir Guri No edit summary current
• 19:0619:06, 13 July 2010 diff hist +1,138 N User:Yakir Guri No edit summary current