User contributions for Sadao Ota

20 May 2009

• 19:4419:44, 20 May 2009 diff hist +5 SBB09Ntbk-Sadao Ota →Take out the supernatents, getting rid of background of cells current
• 19:4319:43, 20 May 2009 diff hist 0 SBB09Ntbk-Sadao Ota No edit summary
• 19:4319:43, 20 May 2009 diff hist +1 SBB09Ntbk-Sadao Ota →take out the supernatents, getting rid of background of cells
• 19:4119:41, 20 May 2009 diff hist +7 SBB09Ntbk-Sadao Ota No edit summary
• 19:4019:40, 20 May 2009 diff hist +153 SBB09Ntbk-Sadao Ota →Wednesday:
• 19:3719:37, 20 May 2009 diff hist +203 SBB09Ntbk-Sadao Ota →Sadao 14:02, 6 May 2009 (EDT)
• 19:3319:33, 20 May 2009 diff hist −34 SBB09Ntbk-Sadao Ota →Wednesday:
• 19:3019:30, 20 May 2009 diff hist +2,126 SBB09Ntbk-Sadao Ota No edit summary
• 19:2519:25, 20 May 2009 diff hist −25 SBB09Ntbk-Sadao Ota →Results from incubation on Monday
• 19:2419:24, 20 May 2009 diff hist +7 SBB09Ntbk-Sadao Ota →Results from incubation on Monday
• 19:2319:23, 20 May 2009 diff hist +535 SBB09Ntbk-Sadao Ota →Sadao 14:02, 29 April 2009 (EDT)
• 19:2119:21, 20 May 2009 diff hist +333 SBB09Ntbk-Sadao Ota No edit summary
• 19:2019:20, 20 May 2009 diff hist +964 SBB09Ntbk-Sadao Ota No edit summary
• 19:1519:15, 20 May 2009 diff hist +10 SBB09Ntbk-Sadao Ota →Sadao 14:02, 27 April 2009 (EDT)
• 19:1119:11, 20 May 2009 diff hist +110 SBB09Ntbk-Sadao Ota No edit summary

16 May 2009

• 05:5605:56, 16 May 2009 diff hist −189 SBB09Ntbk-Sadao Ota →Sadao 13:20, 27 April 2009 (EDT)

29 April 2009

• 10:1510:15, 29 April 2009 diff hist −10 SBB09Ntbk-Sadao Ota No edit summary
• 10:1410:14, 29 April 2009 diff hist −220 SBB09Ntbk-Sadao Ota No edit summary
• 10:1410:14, 29 April 2009 diff hist +2,260 SBB09Ntbk-Sadao Ota No edit summary
• 10:0510:05, 29 April 2009 diff hist −10 SBB09Ntbk-Sadao Ota →Sadao 13:20, 22 April 2009 (EDT)

22 April 2009

• 19:2619:26, 22 April 2009 diff hist +882 SBB09Ntbk-Sadao Ota No edit summary
• 19:2019:20, 22 April 2009 diff hist −3 SBB09Ntbk-Sadao Ota →Sadao 13:41, 20 April 2009 (EDT)
• 19:1919:19, 22 April 2009 diff hist +38 SBB09Ntbk-Sadao Ota →Preparation of Tris buffer solution
• 19:1319:13, 22 April 2009 diff hist +2,448 SBB09Ntbk-Sadao Ota No edit summary

9 March 2009

• 19:3719:37, 9 March 2009 diff hist +98 SBB09 seq No edit summary
• 18:4618:46, 9 March 2009 diff hist +46 SBB09 seq No edit summary

6 March 2009

• 21:1421:14, 6 March 2009 diff hist +22 SBB09 seq No edit summary

4 March 2009

• 20:1120:11, 4 March 2009 diff hist −2 SBB09 seq No edit summary
• 20:1020:10, 4 March 2009 diff hist +7 SBB09 seq No edit summary

25 February 2009

• 20:1120:11, 25 February 2009 diff hist +354 N SBB09 Ligation of EcoRI/BamHI digests New page: *Set up the following reaction: 6.5uL ddH2O 1uL T4 DNA Ligase Buffer (small red or black-striped tubes) 1uL Vector digest 1uL Insert digest 0.5uL T4 DNA Ligase *Pound upside down... current
• 20:1120:11, 25 February 2009 diff hist −310 SBB09Ntbk-Sadao Ota No edit summary
• 20:0920:09, 25 February 2009 diff hist +4 SBB09Ntbk-Sadao Ota →Sadao Ota 15:03, 23 February 2009 (EST)
• 20:0820:08, 25 February 2009 diff hist −406 SBB09Ntbk-Sadao Ota No edit summary
• 20:0720:07, 25 February 2009 diff hist +405 N SBB09 PCR Cloning New page: '''Dilute oligonucleotides to be 10 uM''' * Add 1 ul of oligo dilution to 9 ul of ddH20 * Mix and spin '''Cloning PCR, (products are 937bp & 1600bp, )''' * 24 uL water * 3.3 uL Expand 10... current
• 20:0720:07, 25 February 2009 diff hist +43 SBB09Ntbk-Sadao Ota No edit summary
• 20:0420:04, 25 February 2009 diff hist −52 SBB09Ntbk-Sadao Ota No edit summary
• 20:0420:04, 25 February 2009 diff hist +357 N SBB-Protocols Digest New page: *Set up the following reaction: 8uL of eluted PCR product 1uL of NEB Buffer 2 0.5uL EcoRI 0.5uL BamHI *Incubate at 37 degrees on the thermocycler for 1hr *Clean Up again as describ... current
• 20:0420:04, 25 February 2009 diff hist −327 SBB09Ntbk-Sadao Ota No edit summary
• 20:0220:02, 25 February 2009 diff hist +49 N SBB-Protocols Gelation Ana New page: mix the eluted DNAs (3 ul) and loading dye (2 ul) current
• 20:0220:02, 25 February 2009 diff hist +37 SBB09Ntbk-Sadao Ota No edit summary
• 19:5919:59, 25 February 2009 diff hist +478 N SBB-Protocols Zymo1 New page: #Add 180 uL of Zymo ADB buffer (brown bottle) to a 33uL or 50uL reaction. #Transfer into the Zymo column (small clear guys) #spin through, discard waste. #Add 200 uL of PE or Zymo Wash buf... current
• 19:5919:59, 25 February 2009 diff hist −478 SBB09Ntbk-Sadao Ota No edit summary
• 19:5819:58, 25 February 2009 diff hist +179 SBB09Ntbk-Sadao Ota No edit summary
• 18:4118:41, 25 February 2009 diff hist +458 SBB09Ntbk-Sadao Ota No edit summary

24 February 2009

• 06:2206:22, 24 February 2009 diff hist −71 SBB09Ntbk-Sadao Ota No edit summary
• 06:2106:21, 24 February 2009 diff hist +157 SBB09Ntbk-Sadao Ota →Sadao Ota 15:03, 23 February 2009 (EST)
• 06:2006:20, 24 February 2009 diff hist −320 SBB09Ntbk-Sadao Ota No edit summary
• 06:1806:18, 24 February 2009 diff hist +511 SBB09Ntbk-Sadao Ota No edit summary
• 06:1306:13, 24 February 2009 diff hist +3 SBB09Ntbk-Sadao Ota No edit summary
• 06:1106:11, 24 February 2009 diff hist +1,114 SBB09Ntbk-Sadao Ota No edit summary