User contributions for Nrj5011

17 September 2008

• 01:0401:04, 17 September 2008 diff hist +202 N IGEM:PennState/Labbook/GarrettTobin08/2008/09 Autocreate Month 2008/09 Entry for IGEM:PennState/Labbook/GarrettTobin08 current
• 01:0401:04, 17 September 2008 diff hist +975 N IGEM:PennState/Labbook/GarrettTobin08/2008/09/17 Autocreate 2008/09/17 Entry for IGEM:PennState/Labbook/GarrettTobin08

15 April 2008

• 19:5119:51, 15 April 2008 diff hist +77 N IGEM:PennState/Labbook/NoahJohnson/2008-4-15 New page: <h1>April 4, 2007</h1> *Replated all fridge plates *Made plates (60 amp) 2- current
• 19:5019:50, 15 April 2008 diff hist +140 IGEM:PennState/Labbook/NoahJohnson No edit summary current

26 February 2008

• 23:2023:20, 26 February 2008 diff hist +54 N IGEM:PennState/Labbook/NoahJohnson/2008-2-26 New page: <h1>Feb. 26, 2007</h1> *electrocomp. cell prep 4:45- current

23 February 2008

• 01:2301:23, 23 February 2008 diff hist +4 IGEM:PennState/Labbook/NoahJohnson/2008-2-21 No edit summary current
• 01:2301:23, 23 February 2008 diff hist +53 N IGEM:PennState/Labbook/NoahJohnson/2008-2-22 New page: <h1>Feb. 22, 2008</h1> *Finished replating 7:30-8:30 current

21 February 2008

• 22:1622:16, 21 February 2008 diff hist +135 N IGEM:PennState/Labbook/NoahJohnson/2008-2-21 New page: <h1>Feb. 21, 2008</h1> *Made plates **20 LB amp **20 LB kan *Replated all fridge plates *Autoclaved tips and contaminated media 3:30-

27 January 2008

• 19:4819:48, 27 January 2008 diff hist −32 IGEM:PennState/Labbook/NoahJohnson/2008-1-27 Removing all content from page current
• 19:4419:44, 27 January 2008 diff hist +32 N IGEM:PennState/Labbook/NoahJohnson/2008-1-27 New page: <h1>January 27, 2008</h1> 2:30-

11 January 2008

• 19:0119:01, 11 January 2008 diff hist +81 N IGEM:PennState/Labbook/NoahJohnson/2008-1-6 New page: <h1>January 6, 2007</h1> *Worked on presentation *took 48hr timepoint 12-2, 9-11 current
• 18:5418:54, 11 January 2008 diff hist +32 N IGEM:PennState/Labbook/NoahJohnson/2007-12-27 New page: <h1>December 27, 2007</h1> 12-7 current

6 January 2008

• 17:4817:48, 6 January 2008 diff hist +79 N IGEM:PennState/Labbook/NoahJohnson/2008-1-5 New page: <h1>January 6, 2007</h1> *Took 24h data point *Ran samples in IC overnight 1-3 current
• 17:4617:46, 6 January 2008 diff hist +5 IGEM:PennState/Labbook/NoahJohnson/2008-1-4 No edit summary current

4 January 2008

• 21:0921:09, 4 January 2008 diff hist +52 N IGEM:PennState/Labbook/NoahJohnson/2008-1-4 New page: <h1>January 4, 2008</h1> *fermentation test run 11-
• 21:0821:08, 4 January 2008 diff hist +29 IGEM:PennState/Labbook/NoahJohnson/2008-1-3 No edit summary current

3 January 2008

• 20:1120:11, 3 January 2008 diff hist +78 N IGEM:PennState/Labbook/NoahJohnson/2008-1-3 New page: <h1>January 3, 2007</h1> *Innoculated W3110 for fermentation run tomorrow 10-
• 20:1020:10, 3 January 2008 diff hist +59 N IGEM:PennState/Labbook/NoahJohnson/2008-1-2 New page: <h1>January 2, 2007</h1> *Made LB Amp plates *Replated 1-6 current

30 December 2007

• 18:4618:46, 30 December 2007 diff hist +6 IGEM:PennState/Labbook/NoahJohnson/2007-12-30 No edit summary current
• 16:4916:49, 30 December 2007 diff hist +26 N IGEM:PennState/Labbook/NoahJohnson/2007-12-30 New page: <h1>December 30, 2007</h1>

29 December 2007

• 17:4317:43, 29 December 2007 diff hist +100 Nm IGEM:PennState/Labbook/NoahJohnson/2007-12-29 New page: <h1>December 29, 2007</h1> *Replated all plates in fridge *Reinnoculated for test run tomorrow 10-1 current

28 December 2007

• 22:0322:03, 28 December 2007 diff hist +94 N IGEM:PennState/Labbook/NoahJohnson/2007-12-28 New page: <h1>December 28, 2007</h1> *Made M9 min media *Prepped for test fermentation run tomorrow 1-5 current

21 December 2007

• 15:3915:39, 21 December 2007 diff hist 0 IGEM:PennState/Labbook/NoahJohnson No edit summary
• 15:3915:39, 21 December 2007 diff hist 0 IGEM:PennState/Labbook/NoahJohnson No edit summary
• 15:3815:38, 21 December 2007 diff hist +140 IGEM:PennState/Labbook/NoahJohnson No edit summary

20 November 2007

• 21:0721:07, 20 November 2007 diff hist +117 N IGEM:PennState/Labbook/NoahJohnson/2007-11-20 New page: <h1> Nov. 20, 2007 </h1> *Submitted crp* for sequencing *made LB Amp, and LB Kan plates *replated fridge plates 12-5 current

31 October 2007

• 05:4905:49, 31 October 2007 diff hist +48 N IGEM:PennState/Labbook/NoahJohnson/2007-10-30 New page: <h1>October 30, 2007</h1> *Worked on poster 4-9 current
• 05:4805:48, 31 October 2007 diff hist +78 N IGEM:PennState/Labbook/NoahJohnson/2007-10-29 New page: <h1> October 29, 2007</h1> *Group Meeting for practice presentation 4:30-5:30 current

30 October 2007

• 22:0222:02, 30 October 2007 diff hist +56 N IGEM:PennState/Labbook/NoahJohnson/2007-10-27 New page: <h1> October 27, 2007</h1> *Worked on presentation 4-12 current

26 October 2007

• 02:4402:44, 26 October 2007 diff hist +56 N IGEM:PennState/Labbook/NoahJohnson/2007-10-25 New page: <h1>October 25, 2007</h1> *worked on website 5:30-10:30 current

25 October 2007

• 23:4323:43, 25 October 2007 diff hist 0 IGEM:PennState/2007/Lab-photos No edit summary current
• 23:4223:42, 25 October 2007 diff hist −111 IGEM:PennState/2007/Lab-photos No edit summary
• 23:4123:41, 25 October 2007 diff hist −207 IGEM:PennState/2007/Lab-photos No edit summary
• 15:0915:09, 25 October 2007 diff hist +81 N IGEM:PennState/Labbook/NoahJohnson/2007-10-22 New page: <h1>October 25, 2007</h1> *Group meeting @ 4pm *Replated fridge plates 3:30-6:30 current
• 02:2902:29, 25 October 2007 diff hist +311 User:Nrj5011 No edit summary current

18 October 2007

• 21:3321:33, 18 October 2007 diff hist +4 IGEM:PennState/Labbook/NoahJohnson/2007-10-16 No edit summary current
• 21:3321:33, 18 October 2007 diff hist +139 N IGEM:PennState/Labbook/NoahJohnson/2007-10-18 New page: <h1>October 18, 2007</h1> *Picked up primers (1457 & 1458) **1457: pSB1A2 Forward **1458: pSB1A2 Reverse *Sent in crp* for sequencing 4- current

16 October 2007

• 21:1621:16, 16 October 2007 diff hist +118 IGEM:PennState/Labbook/NoahJohnson/2007-10-16 No edit summary
• 16:2216:22, 16 October 2007 diff hist +141 N IGEM:PennState/Labbook/NoahJohnson/2007-10-16 New page: <h1>October 16, 2007</h1> *Filled pipet tip boxes *Miniprepped crp* in psB1A2 *Replated crp* from overnight culture on two LB Amp plates 12-

15 October 2007

• 22:0722:07, 15 October 2007 diff hist +151 N IGEM:PennState/Labbook/NoahJohnson/2007-10-15 New page: <h1>October 15, 2007</h1> *Innoculated from transformation plate *Re-plated working transformation *Filled pipet-tip boxes *Group meeting @5 3:30-5:30 current
• 01:2201:22, 15 October 2007 diff hist +70 IGEM:PennState/Labbook/NoahJohnson/2007-10-14 No edit summary current

14 October 2007

• 17:0917:09, 14 October 2007 diff hist +79 N IGEM:PennState/Labbook/NoahJohnson/2007-10-14 New page: <h1>October 14, 2007</h1> *Made LB Amp plates (40) *Electrocomp. cell prep 12-

11 October 2007

• 23:2423:24, 11 October 2007 diff hist +133 N IGEM:PennState/Labbook/NoahJohnson/2007-10-11 New page: <h1>October 11, 2007</h1> *Ran restriction products on a gel *Gel extracted crp* and psB1A2 *crp* & psB1A2 overnight ligation 4:30-8 current
• 20:4320:43, 11 October 2007 diff hist +84 N IGEM:PennState/Labbook/NoahJohnson/2007-10-10 New page: <h1>October 10, 2007</h1> *E+S overnight restriction digest on pSB1A2 and crp* 9-10 current

10 October 2007

• 13:1813:18, 10 October 2007 diff hist +83 N IGEM:PennState/Labbook/NoahJohnson/2007-10-9 New page: <h1>October 10, 2007</h1> *Electroporation transformation in Dr. Cirino's lab 4-10 current

9 October 2007

• 04:4504:45, 9 October 2007 diff hist +58 IGEM:PennState/Labbook/NoahJohnson/2007-10-8 No edit summary current

8 October 2007

• 23:2823:28, 8 October 2007 diff hist +2 IGEM:PennState/Labbook/NoahJohnson/2007-10-8 No edit summary
• 23:0223:02, 8 October 2007 diff hist +83 N IGEM:PennState/Labbook/NoahJohnson/2007-10-7 New page: <h1>October 7, 2007</h1> *Transformed via electroporation in Dr.Cirino's lab 7-10 current
• 23:0023:00, 8 October 2007 diff hist +122 N IGEM:PennState/Labbook/NoahJohnson/2007-10-8 New page: <h1>October 8, 2007</h1> *Made 20 tubes of Amp stock solution **100mg/mL, 1mL in each tube *group meeting @ 5:30 4:30-7,

30 September 2007

• 23:3923:39, 30 September 2007 diff hist +79 N IGEM:PennState/Labbook/NoahJohnson/2007-9-24 New page: <h1>September 24, 2007</h1> *Cleaned up in lab *group meeting @ 4:30 3:30-5:30 current