User contributions for Colin P. McNally

13 August 2011

• 00:3500:35, 13 August 2011 diff hist +55 McClean:Protocols →Flow Cytometry
• 00:3200:32, 13 August 2011 diff hist +1,206 N McClean:Phase Plane Mapping New page: ==Purpose== Expose a strain of yeast to all combinations of two ranges of stimulants, in this case alpha-factor and sorbitol, and measure their response with fluorescent markers and flow c... current

12 August 2011

• 21:1721:17, 12 August 2011 diff hist +58 McClean:Protocols →Flow Cytometry
• 21:1221:12, 12 August 2011 diff hist +285 McClean:Induction Timecourse No edit summary current
• 20:0120:01, 12 August 2011 diff hist +1,232 N McClean:Induction Timecourse New page: #Fill falcon tubes with 800 microL PBS + .1% tween, put on ice #Grow up 25ml of yMM736 to mid-log phase in a klett flask (aim for klett 50-60). Have one klett flask for each condition to ...
• 19:0419:04, 12 August 2011 diff hist +77 McClean:Protocols →Flow Cytometry
• 19:0319:03, 12 August 2011 diff hist +916 N McClean:Cycloheximide Concentration New page: ==Purpose== Find the minimum concentration of cyclohexamide required to fully halt translation in S. cerevisae. ==Protocol== #Fill falcon tubes with 800 microL PBS + .1% tween, put on i... current
• 18:5518:55, 12 August 2011 diff hist +14 McClean: Fluorescent Folding Time Assay →Notes current
• 18:5418:54, 12 August 2011 diff hist +439 McClean: Fluorescent Folding Time Assay →Protocol
• 18:4818:48, 12 August 2011 diff hist +3 McClean: Fluorescent Folding Time Assay →Overview
• 18:3818:38, 12 August 2011 diff hist +502 McClean: Fluorescent Folding Time Assay →Overview
• 18:3718:37, 12 August 2011 diff hist +1,241 N McClean:Fluorescent Protein Start New page: ==Purpose== Find the earliest detectable fluorescent protein after induction. ==Protocol== #Fill falcon tubes with 800 microL PBS + .1% tween, put on ice #Grow up 25ml of yMM736 to mid-... current
• 16:4616:46, 12 August 2011 diff hist +4 McClean:FISH →Protocol
• 16:3916:39, 12 August 2011 diff hist +5,441 McClean:FISH →Protocol

11 August 2011

• 20:2420:24, 11 August 2011 diff hist +24 N McClean:FISH New page: ==Protocol== #Grow cells
• 20:2320:23, 11 August 2011 diff hist +56 McClean:Protocols →Yeast
• 20:2220:22, 11 August 2011 diff hist +661 McClean: Fluorescent Folding Time Assay No edit summary
• 19:0919:09, 11 August 2011 diff hist +7 McClean:General Flow Cytometry Procedure →Procedure

9 August 2011

• 21:1021:10, 9 August 2011 diff hist +91 McClean:Protocols →FACS
• 21:0921:09, 9 August 2011 diff hist +392 N McClean:General Flow Cytometry Procedure New page: ==Materials== #PBS + 0.1% Tween 20 #Falcon 2058 6mL tubes #a sonicator #BD LSRII ==Procedure== #For each sample fill a falcon tube with 0.8mL of PBS + 0.1% Tween and put it on ice #Grow y...
• 20:5020:50, 9 August 2011 diff hist +14 McClean: Fluorescent Folding Time Assay No edit summary
• 19:5019:50, 9 August 2011 diff hist +85 McClean:Protocols →FACS
• 19:4819:48, 9 August 2011 diff hist +55 Nm McClean: Fluorescent Folding Time Assay New page: ==Materials== #yeast #liquid media #tubes #FACS machine
• 19:4219:42, 9 August 2011 diff hist +10 McClean:Protocols No edit summary
• 19:3519:35, 9 August 2011 diff hist −29 User:Colin P. McNally →Research interests current
• 19:3419:34, 9 August 2011 diff hist −5 User:Colin P. McNally →Contact Info

27 July 2011

• 17:5617:56, 27 July 2011 diff hist −45 User:Colin P. McNally →Education
• 17:5117:51, 27 July 2011 diff hist +3,212 N User talk:Colin P. McNally No edit summary current
• 17:5117:51, 27 July 2011 diff hist +1,190 N User:Colin P. McNally No edit summary