User contributions for Christina Schlesinger

17:2117:21, 16 April 2013 diff hist +30 SSB13Ntbk - Apr16 →Golden Gate Assembly current
17:2017:20, 16 April 2013 diff hist +130 N SSB13Ntbk - Apr16 New page: ==Golden Gate Assembly== 3.5 μl ddH2O 1 μl Ligase Buffer .5 μl Ligase .5 μl BsmB1 1 μl per construct (4 total) .5 μl vector
17:1817:18, 16 April 2013 diff hist +44 SBB13Ntbk-ChristinaS No edit summary current

22:0022:00, 2 April 2013 diff hist +160 N SSB13Ntbk - Apr2 New page: Got colonies from the transformation performed two weeks ago. Class redid their restriction digest and we all ran our saved samples on the gel. I saw my digest. current
21:5921:59, 2 April 2013 diff hist +42 SBB13Ntbk-ChristinaS No edit summary

17:5617:56, 21 March 2013 diff hist −129 SSB13Ntbk - Mar21 No edit summary current
16:5716:57, 21 March 2013 diff hist +965 N SSB13Ntbk - Mar21 New page: ===Ligation of EcoRI/BamHI digests=== *Set up the following reaction: 6.5uL ddH2O 1uL T4 DNA Ligase Buffer (small red or black-striped tubes) 1uL Vector digest 1uL Insert digest ...
16:3916:39, 21 March 2013 diff hist +44 SBB13Ntbk-ChristinaS No edit summary
07:0907:09, 21 March 2013 diff hist +1,260 N SSB13Ntbk - Mar19 New page: ===Regular Zymo Cleanup=== #Add 180 uL of Zymo ADB buffer to PCR reaction. #Transfer into the Zymo column #spin through, discard waste. #Add 200 uL of Zymo Wash Buffer #spin through, disc... current
07:0407:04, 21 March 2013 diff hist +22 SBB13Ntbk - Mar14 No edit summary current
07:0307:03, 21 March 2013 diff hist +953 N SBB13Ntbk - Mar14 New page: The PCA amplification did not yield enough PCR product, so need to do re-amplification. #Ran an analytical gel, 1%, 10ul of PCR product. Thankfully saw PCR product, but a very small amoun...
06:5806:58, 21 March 2013 diff hist +166 SBB13Ntbk - Mar12 No edit summary current
06:5506:55, 21 March 2013 diff hist −13 SBB13Ntbk-ChristinaS No edit summary
06:5506:55, 21 March 2013 diff hist +13 SBB13Ntbk-ChristinaS No edit summary
06:5306:53, 21 March 2013 diff hist +88 SBB13Ntbk-ChristinaS No edit summary

17:0317:03, 12 March 2013 diff hist +512 N SBB13Ntbk - Mar12 New page: ==Regular Zymo Cleanup== #Add 180 uL of Zymo ADB buffer (brown bottle) to a 33uL or 50uL reaction. #Transfer into the Zymo column (small clear guys) #spin through (top speed, 30 sec), dis...
16:5216:52, 12 March 2013 diff hist +1 SBB13Ntbk - Mar8 No edit summary current
16:5216:52, 12 March 2013 diff hist +24 SBB13Ntbk - Mar8 No edit summary
16:5116:51, 12 March 2013 diff hist +178 N SBB13Ntbk - Mar8 New page: ==PCA Assembly - Part 1==<br> PCA mixture 38 uL ddH2O 5 ul 10x expand buffer 5 ul 2mM dNTPs 1 ul oligo mixture (100mM) .75 Expand polymerase gave the mix to Anderson to run PCA
16:4716:47, 12 March 2013 diff hist +86 SBB13Ntbk-ChristinaS No edit summary
16:4616:46, 12 March 2013 diff hist +5,801 N SBB13Ntbk - Mar7 New page: <pre> PCA quiC-4 - oligo1-16 (464bp, pca1) PCR pca1 with quiC-NewForward/Reverse (418bp, pca2) Digest pca2 (EcoRI/BglII, 402+10+6,L,... current
16:4516:45, 12 March 2013 diff hist −5,803 SBB13Ntbk-ChristinaS Replacing page with ' March 7, 2013 <br>'
16:4516:45, 12 March 2013 diff hist +41 SBB13Ntbk-ChristinaS No edit summary
16:4316:43, 12 March 2013 diff hist +2 SBB13Ntbk-ChristinaS No edit summary

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