Smolke:Protocols/Media stocks

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  • When aliquoting media stock make sure to leave some empty volume in the tube (i.e., fill a 50-ml Falcon to only the 40-ml mark)
    • This is especially important for trypsin and FBS aliquots, which will expand when frozen.
  • Each researcher should have their own aliquoted stock of media.
  • Take responsibility for aliquoting new stocks, when older stocks are almost used up. Make sure to use sterile technique in preparing aliquots.