Smolke:Protocols/Heat Shock Transformation

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For each sample 1 tube of thawed chemically competent cells (keep covered in ice at all times) 1-10 uL ligation product 250 uL SOC 1 LB plate with appropriate antibiotics Procedure Add DNA to cells Incubate on ice for 0-20 minutes Heat shock at 42 °C for 30 seconds Incubate on ice for 0-2 minutes Add SOC Incubate on rotator in 37 °C incubator for 0-60 minutes Plate 50-250 uL