Rules
- Two restriction enzyme coolers are in -20C over the Quanta bay, one labeled common.
- Only common enzymes may have backup, which are stored in main -20C.
- Common enzymes: AgeI, AleI, ApaI, AvrII, BamHI, DpnI, EcoRI, EcoRV, HindIII, MfeI, NotI, SacI, SacII, SalI, XbaI, XhoI
- The enzymes not listed are in the non-common enzyme cooler.
- If the enzyme is available in HF form, only the HF form will be in the backup unless otherwise requested.
- When the working stock has been used up, replace with a new tube from the backup cooler and write the enzyme on the whiteboard.
- Backup ligases and CIP are also in the backup cooler in main -20C.
Restriction Enzymes in Stocks
| Enzyme |
Regular |
HF |
Regular Backup |
HF Backup
|
| AflII |
Y |
|
|
|
| AgeI |
Y |
Y |
|
Y
|
| AleI |
Y |
|
Y |
|
| ApaI |
Y |
|
YY |
|
| AscI |
Y |
|
|
|
| AsiSI |
Y |
|
Y |
|
| AvrII |
Y |
|
Y |
|
| BamHI |
Y |
Y |
|
Y
|
| BglII |
Y |
|
|
|
| BsaI |
|
Y |
|
|
| BsrGI |
Y |
|
|
|
| BstBI |
Y |
|
|
|
| BstXI |
Y |
|
|
|
| Bsu36I |
Y |
|
|
|
| ClaI |
Y |
|
Y |
|
| DpnI |
Y |
|
Y |
|
| EcoRI |
|
Y |
|
Y
|
| EcoRV |
|
Y |
|
Y
|
| FseI |
Jay's -80 |
|
|
|
| HindIII |
Y |
Y |
Y |
|
| KasI |
|
|
|
Y
|
| KpnI |
Y |
Y |
|
Y
|
| MfeI |
|
Y |
|
Y
|
| NdeI |
Y |
|
|
|
| NheI |
Y |
Y |
|
|
| NotI |
Y |
Y |
|
Y
|
| NruI |
Y |
|
|
|
| PacI |
Y |
|
|
|
| PmeI |
Y |
|
|
|
| PstI |
Y |
|
|
|
| PvuII |
|
Y |
|
|
| SacI |
|
Y |
|
Y
|
| SacII |
Y |
|
Y |
|
| SalI |
Y |
Y |
|
Y
|
| SapI |
Y |
|
|
|
| SbfI |
|
Y |
|
|
| SpeI |
Y |
|
Y |
|
| SphI |
|
Y |
|
|
| SwaI |
Y |
|
|
|
| XbaI |
Y |
|
Y |
|
| XhoI |
Y |
|
Y |
|
| XmaI |
Y |
|
|
|
Useful Links
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