Silver: ssDNA

(10 mg/ml)

• ssDNA is stored in cold box (dessicated)
• weigh out enough for 10 mg/ml in dH₂O
• stir at room temperature until dissolved (takes a long time)
• shear DNA by passing 6 times through an 18-gauge needle and 6 times through a 22-gauge needle
• bring up to 3x volume with dH₂0, and then boil solution until it is back at 1x volume.
• aliquot into 1.5 ml Eppendorf tubes and store at –20 °C.