Open main menu

OpenWetWare β

SYBR Gold

Recommended by Molecular Cloning as the best of the SYBR dyes from Molecular Probes.

General information

  • ten-fold more sensitive than ethidium bromide.
  • greater dynamic range.
  • penetrates gels quickly.
  • Stains both DNA and RNA in conventional neutral polyacrylamide and agarose gels and in denaturing gels using urea, glyoxal or formaldehyde.
  • stain gels post-electrophoresis because SYBR Gold likely binds the charge phosphate backbone of nucleic acids altering the electrophoretic mobility significantly. (DNA bands can come out curved).
  • low background so no destaining is necessary.
  • can be excited at by standard transillumination at 300nm.
  • stained nucleic acids can be transferred to membranes for Northerns or Southerns.
  • doesn't inhibit most enzymatic reactions except PCR. PCR is sensitive to high dye concentrations. Address this issues by adjusting Mg2+ concentration or remove stain via ethanol precipitation.
  • very expensive
  • supplied at 10,000X concentration in anhydrous DMSO.
  • operates most efficiently between pH 7-8.5
  • stock solution is stable 6 months to 1 year at <= 20°C

References