# STEM Facility

THE STEM (Scanning Transmission Electron Microscope) FACILITY

       in the Biology Deprtment at Brookhaven National Laboratory


The STEM was an NIH Research Resource and as such was available to outside users with appropriate projects free of charge.

After we lost our STEM grant, we have been operating, halftime, on a Fee-for-Service basis. We had twenty-five outside users (outside BNL) this year (2008). It was a particularly productive year for measuring the mass per length (M/L) of filaments (amyloid, prions, and DNA) to determine their structure and composition.

The microscope is now running with our new computer system. A new, complete, PCMass program is on our ftp site (ftp.stem.bnl.gov)with new tutorials.

Our web page is being revised. (see it (reload or refresh) http://www.biology.bnl.gov/stem/stem.html) It now has almost everything on it, but is still undergoing some revisions. A brief summary of the Fee-for-Service is below:

FEE-FOR-SERVICE

Details on our web page include:

• Services Provided
• Guarantee

SAMPLE CHARGE

Biology’s Scanning Transmission Electron Microscope (STEM)

Per Grid* – non-proprietary…………………………..\$391.02**

Minimum of 3 grids per specimen

Our web page (link given above) has essentially all the useful information about the facility and its use.

We will try to use this page for transient information, such as announcements and "news".

USERS

Potential users (from January on) should contact either Joe or Beth. Probably e-mail is better than the telephone. Joe and Beth will keep the User Program running, on a half-time basis.

NEWS

Martha Simon and Frank Kito retired January 2, 2009. Jim Hainfeld left BNL at the end of February, 2009 and is currently at Nanoprobes.

ANNUAL EM WORKSHOP

No annual STEM/EM Workshop was held in 2008 at the end of the RapiData Crystallography course.