SR

Summary

Super Rich Medium (aka SR Medium). This medium is optimized for high-yield protein expression in Escherichia coli.

Ingredients

Solution A

1. 20 g tryptone
2. 20 g yeast extract
3. 10 g beef extract
4. 11 g glycerol
5. 900 mL H₂O

Solution B

1. 2.32 g KH₂PO₄
2. 16.4 g K₂HPO₄*H₂O
3. 100 mL H₂O

Protocol

1. Dissolve ingredients of both solutions.
2. Pour into flasks or bottles (Solution A in 2 L flask or greater, Solution B in 250 mL flask or greater).
3. Autoclave (liquid cycle)

Source

Feil, I. (1992) PH.D. Thesis, Technische Universtiaet Carolo-Wilhelmina zu Braunschweig, Braunschweig, Germany