SPR surface regeneration and wash conditions

surface	procedure type	purpose	conditions	references
CM5, streptavidin covered + biotinylated DNA	regeneration	removal of analyte proteins	100-µl pulse of 1% SDS in 10 mM HEPES, flow rate 100 µl/min	[1]

1. Maesawa C, Inaba T, Sato H, Iijima S, Ishida K, Terashima M, Sato R, Suzuki M, Yashima A, Ogasawara S, Oikawa H, Sato N, Saito K, and Masuda T. A rapid biosensor chip assay for measuring of telomerase activity using surface plasmon resonance. Nucleic Acids Res. 2003 Jan 15;31(2):E4-4. DOI:10.1093/nar/gng004 |