SOB Media
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| Reagent/Final Vol | 400 ml | 800 ml | 1600 ml |
|---|---|---|---|
| Tryptone | 8 g | 16 g | 32 g |
| Yeast | 2 g | 4 g | 8 g |
| NaCl | 0.2 g | 0.4 g | 0.8 g |
| 0.25 M KCl (add after dissolving the foregoing) | 4 ml | 8 ml | 16 ml |
pH 7.0 with 5 N NaOH. Autoclave. Add 2 mls filter sterilized 2M MgCl2 to each 400 ml.
