SEED/2009/Day 7

Morning

• Discuss sequencing technologies
• Review labs so far

Afternoon

• pick 1 blue/green colony per plate for growth and templiphi in strips
  • pick 4 colonies per group
  • 2.5ul sample buffer per tube
  • pick colony and dip in tube
  • 3m@95C to break open cells
  • Add 2.5ul reaction buffer + 0.1ul enzyme mix
• incubate templiphi at 30C overnight
• Class project mini-presentations/discussions

Instructor Post-prep

• heat kill templiphi
• transfer 2ul of templiphi into sequencing mixes with VF2
• Send out for sequencing