SBB12 gels

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SynBioBootcamp Homepage
How to Load, Run, and Upload Gels

Ladder

Unless otherwise noted, we will be using the NEB 2-log ladder. NEB site
NEB 2-log ladder.gif

Example

Invitrogen1kbplus.jpg

JCAnderson 14:40, 17 February 2011 (EST)

021711-AnalGel1 (just an example)
021711-AnalGel1.jpg
This one used 1kb plus.



Gels: Feb 17

MW ladder used in these gels

MW-ladder.jpg

Au Duong 13:35, 17 February 2012 (EST)

ClassGel1 2_17_2012
Gel1 from class 2 17 2012.jpg

Zachary Russ 13:43, 17 February 2012 (EST)

ClassGel2 Gel2 from class 2 17 2012.jpg

Lanes:

1 2 3 4 5 6 7 8 9 10
JW A JW B MM empty MW ladder JS DC A DC B empty empty


Gels: Feb 21

Tina Nie 13:56, 21 February 2012 (EST)

Class Gel 1
2012 02 21 gel1 ssb2012spring.jpg

Lanes:

1 2 3 4 5 6 7 8 9 10
Au PCA2 TN sbb1213 DC A DC B 2-log ladder JS MM JW RO TP RO CT

Tina Nie 14:10, 21 February 2012 (EST)

ClassGel2
2012 02 21 gel2 ssb2012spring.jpg

Lanes:

1 2 3 4 5 6 7 8 9 10
LZ AAAA PCA2 sbb1224 sbb1203 2-log ladder AJ pca2 MD JK caff-PCA2 AJ A AJ B PR PCR1


Gels: Feb 23

Class Gel #1
NEB 2-log ladder.gif2012 02 23 gel1 ssb2012spring.jpg

Lanes:

1 2 3 4 5 6 7 8 9 10
MM PR-PCA JS Tar dig 2-log ladder Jkotker LZ (1204) MD PhoA sbb1224 MD LZ MH PCR A+B

Class Gel #2
NEB 2-log ladder.gif2012 02 23 gel2 ssb2012spring lo.jpg2012 02 23 gel2 ssb2012spring hi.jpg

Lanes:

1 2 3 4 5 6 7 8 9 10
GF 438 GF 2801 empty JKotker Caff (1223) empty 2-log ladder PR-PCR Karen PCRproduct empty Au SOE digest

Gels: Feb 24

Gel 1

NEB 2-log ladder.gif2012 02 24 gel1 ssb2012spring.jpg

Lanes:

1 2 3 4 5 6 7 8 9 10
MH PCRPDT 1218 MH PCRDIG 1218 empty DC SB 1221 Ladder empty empty empty GF PCA RO SOE3 1231

Gels: Feb 28

Au Duong 14:19, 28 February 2012 (EST)

ClassGel1 2_28_2012
NEB 2-log ladder.gifGel1 from class 2 28 2012.jpg
\

Robert D O'Dowd 14:19, 28 February 2012 (EST)

Class Gel #2
NEB 2-log ladder.gif2012 02 28 gel2 ssb2012spring.jpg2012 02 28 gel2 ssb2012spring lo.jpg

Lanes:

1 2 3 4 5 6 7 8 9 10
empty AJ A+B pcrpdt empty RD 1231 dig empty 2-log ladder empty empty empty empty


Gels: Mar 1

Class Gel #1

NEB 2-log ladder.gif2012 03 1 gel1 ssb2012spring.jpg


Class Gel #2

NEB 2-log ladder.gif2012 03 01 gel2 ssb2012spring.jpg

Lanes:

1 2 3 4 5 6 7 8 9 10
Karin 1206 1224(1) 1224(2) 1224(3) 1203(1) ladder AJ RO TM PhoI RO CITM Bad Load


Gels:March 1 (Gel 3)

NEB 2-log ladder.gif2012 03 01 gel3 ssb2012spring.jpg

Lanes:

1 2 3 4 5 6 7 8 9 10
entry one two 1217 four ladder Lz-AAAA1(1) Lz-AAAA1(2 eight nine ten


Gels: Mar 2

Gel 1

NEB 2-log ladder.gif2012 03 02 gel1 ssb2012spring.jpg

Lanes:

1 2 3 4 5 6 7 8 9 10
Au SOE3 AJ A+B empty DC A+B 2log empty empty empty JW A2 2log

Gel 2

NEB 2-log ladder.gif2012 03 02 gel2 ssb2012spring.jpg

Lanes:

1 2 3 4 5 6 7 8 9 10
AJ A+B AJ A+B MM 1232-1 MM 1232-2 MM 1215-2 RO MP 1214 Ladder MDP2 MDP1 MDL2

Gel 3

NEB 2-log ladder.gif2012 03 02 gel3 ssb2012spring.jpg

Lanes:

1 2 3 4 5 6 7 8 9 10
KA miniprep1 1206 KA miniprep2 1206 KA miniprep3 1206 HH Tar 1 2log ladder HH Tar 2 HH LZ 1 HH LZ 2 MH 1207-1 MH 1207-2


Gels: Mar 6

Gel 1

NEB 2-log ladder.gif2012 03 05 gel1 ssb2012spring.jpg

Class Gel 2

NEB 2-log ladder.gif2012 03 06 gel2 ssb2012spring.jpg

Lanes:

1 2 3 4 5 6 7 8 9 10
AJ A+B JS 1201-1 JS 1201-2 JS 1226-1 JS 1226-2 Ladder PR 02-1 PR 02-2 PR 30-1 JK 1233-1


Gel 3

NEB 2-log ladder.gif2012 03 05 gel3 ssb2012spring.jpg

Class Gel 4

NEB 2-log ladder.gif2012 03 06 gel4 ssb2012spring.jpg

Lanes:

1 2 3 4 5 6 7 8 9 10
MM 1232-1 MM 1232-2 MM 1215-2 DC 1221 A+ B Pur Ladder JK 1233-2 JK 1204-1 JK 1204-2 PR 30-2 PR 30-3

Class Gel 5

NEB 2-log ladder.gif2012 03 06 gel5 ssb2012spring.jpg

Lanes:

1 2 3 4 5 6 7 8 9 10
PR 30-4 PR 02-1 PR 02-2 PR 02-3 PR 02-4 Ladder JK 1223-1 AJ 12205-1 AJ 12205-2 AJ 12205-3

Gels: Mar 8

Class Gel 1

NEB 2-log ladder.gif2012 03 08 gel1 ssb2012spring.jpg

Lanes:

1 2 3 4 5 6 7 8 9 10
Ladder 2μL GF PCA Test Ladder 1:5 10μL JW A4 Ladder 3μL LZ AAAA-1 JW A4 1224 M1 1224 M2 MH A+B pcrpdt

Class Gel 2

NEB 2-log ladder.gif2012 03 08 gel2 ssb2012spring.jpg

Class Gel 3

NEB 2-log ladder.gif2012 03 08 gel3 ssb2012spring.jpg2012 03 08 gel3 ssb2012spring hi.jpg

Lanes:

1 2 3 4 5 6 7 8 9 10
AJ Digest 1 AJ Digest 2 AJ Digest 3 AJ Digest 4 Test Ladder 10μL TN sbb1227 3 TN sbb1227 4 JK 1204-1 AJ A+B Digest (cut) Test Ladder 10μL



Gels: Mar 13

Class Gel 1

NEB 2-log ladder.gif2012 03 13 gel1 ssb2012spring.jpg

Lanes:

1 2 3 4 5 6 7 8 9 10
Karen A sbb1217 mp1 Karen A sbb1217 mp2 Karen A sbb1217 mp3 Karen A sbb1217 mp4 Lader sbb1224(1) sbb1224(2) AJ A+B Karen A sbb1217 mp3 Ladder

Class Gel 2

NEB 2-log ladder.gif2012 03 13 gel2 ssb2012spring.jpg

Lanes:

1 2 3 4 5 6 7 8 9 10
DC sbb1221 A+DMSO DC sbb1221 A DC sbb1221 B+DMSO DC sbb1221 B Lader MH 1218 dig JV A5 empty empty empty


Class Gel 3

NEB 2-log ladder.gif2012 03 13 gel3 ssb2012spring.jpg

Lanes:

1 2 3 4 5 6 7 8 9 10
RO 1214 Dig RO 1231 Dig AJ A+B PCR digest **** Lader KA KA KA KA ladder


Gels: Mar 15

Class Gel 1

NEB 2-log ladder.gif2012 03 15 gel1 ssb2012spring.jpg

Class Gel #2

NEB 2-log ladder.gif2012 03 15 gel2 ssb2012spring.jpg

Lanes:

1 2 3 4 5 6 7 8 9 10
sbb1203(1) sbb1203(2) sbb1203(3) sbb1203(4) ladder sbb1224(1) sbb1224(1 sbb1224(2) sbb1224(3) sbb1224(4)


Karen D. Alvarez 15:34, 15 March 2012 (EDT)

Class Gel #3

NEB 2-log ladder.gif2012 03 15 gel3 ssb2012spring.jpg

Lanes:

1 2 3 4 5 6 7 8 9 10
sbb1217(1) sbb1217(2) sbb1217(3) sbb1217(4) ladder ladder -- -- -- --

Gels: March 20

Class Gel 1

NEB 2-log ladder.gif2012 03 20 gel1 ssb2012spring.jpg

Lanes:

1 2 3 4 5 6 7 8 9 10
none MM 1 MM 2 MM 3 MM 4 ladder DC A+B AJ gel purify GF pcrpdt none

Class Gel 2

NEB 2-log ladder.gif2012 03 20 gel2 ssb2012spring.jpg

Lanes:

1 2 3 4 5 6 7 8 9 10
Ladder empty JW BD empty Ladder empty JW A6-B Ladder empty JW A6-B

Class Gel 3

NEB 2-log ladder.gif2012 03 20 gel3 ssb2012spring labeled.jpg


Gels: March 22

Class Gel 1

NEB 2-log ladder.gif2012 03 22 gel1 ssb2012spring.jpg

Lanes:

1 2 3 4 5 6 7 8 9 10
none RO 1214 1 RO 1214 2 RO 1214 3 RO 1214 4 2-log AJ digest DC A+B JW A10 none

Class Gel 2

NEB 2-log ladder.gif2012 03 22 gel2 ssb2012spring.jpg

Lanes:

1 2 3 4 5 6 7 8 9 10
none none none none 2-log DC A+B Digest none none none none